TY - JOUR
T1 - High-performance liquid chromatography of cholesterol autoxidation products
AU - Ansari, G. A.S.
AU - Smith, Leland L.
N1 - Funding Information:
We wish to acknowledge financial support of these studies by the National institutes of Health, U.S. Public Health Service, via research grants ES-00944 and CA-21617.
PY - 1979/7/13
Y1 - 1979/7/13
N2 - High-performance liquid chromatography with microparticulate silica gel and octadecyl silica reversed-phase columns accords ready resolution of the common autoxidation products of cholesterol. Resolution of two pairs of isomeric sterols (5,6α-epoxy-5α-cholestan-3β-ol and 5,6β-epoxy-5β-cholestan-3β-ol, 3β-hydroxy-5α-cholest-6-ene-5-hydroperoxide and 3β-hydroxycholest-5-ene-7α-hydroperoxide) not previously resolved chromatographically has been achieved in both systems. Application of the procedure to the isolation of cholesta-4,6-dien-3-one from autoxidized cholesterol is described.
AB - High-performance liquid chromatography with microparticulate silica gel and octadecyl silica reversed-phase columns accords ready resolution of the common autoxidation products of cholesterol. Resolution of two pairs of isomeric sterols (5,6α-epoxy-5α-cholestan-3β-ol and 5,6β-epoxy-5β-cholestan-3β-ol, 3β-hydroxy-5α-cholest-6-ene-5-hydroperoxide and 3β-hydroxycholest-5-ene-7α-hydroperoxide) not previously resolved chromatographically has been achieved in both systems. Application of the procedure to the isolation of cholesta-4,6-dien-3-one from autoxidized cholesterol is described.
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U2 - 10.1016/S0021-9673(00)89437-6
DO - 10.1016/S0021-9673(00)89437-6
M3 - Article
AN - SCOPUS:0018584177
SN - 0021-9673
VL - 175
SP - 307
EP - 315
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -