High-performance liquid chromatography of cholesterol autoxidation products

Ghulam Ansari, Leland L. Smith

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

High-performance liquid chromatography with microparticulate silica gel and octadecyl silica reversed-phase columns accords ready resolution of the common autoxidation products of cholesterol. Resolution of two pairs of isomeric sterols (5,6α-epoxy-5α-cholestan-3β-ol and 5,6β-epoxy-5β-cholestan-3β-ol, 3β-hydroxy-5α-cholest-6-ene-5-hydroperoxide and 3β-hydroxycholest-5-ene-7α-hydroperoxide) not previously resolved chromatographically has been achieved in both systems. Application of the procedure to the isolation of cholesta-4,6-dien-3-one from autoxidized cholesterol is described.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalJournal of Chromatography A
Volume175
Issue number2
DOIs
StatePublished - Jul 13 1979

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Cholestanol
High performance liquid chromatography
Cholesterol
High Pressure Liquid Chromatography
Silica Gel
Sterols
Silicon Dioxide
Hydrogen Peroxide

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

High-performance liquid chromatography of cholesterol autoxidation products. / Ansari, Ghulam; Smith, Leland L.

In: Journal of Chromatography A, Vol. 175, No. 2, 13.07.1979, p. 307-315.

Research output: Contribution to journalArticle

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