High-performance liquid chromatography of cholesterol autoxidation products

G. A.S. Ansari, Leland L. Smith

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


High-performance liquid chromatography with microparticulate silica gel and octadecyl silica reversed-phase columns accords ready resolution of the common autoxidation products of cholesterol. Resolution of two pairs of isomeric sterols (5,6α-epoxy-5α-cholestan-3β-ol and 5,6β-epoxy-5β-cholestan-3β-ol, 3β-hydroxy-5α-cholest-6-ene-5-hydroperoxide and 3β-hydroxycholest-5-ene-7α-hydroperoxide) not previously resolved chromatographically has been achieved in both systems. Application of the procedure to the isolation of cholesta-4,6-dien-3-one from autoxidized cholesterol is described.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalJournal of Chromatography A
Issue number2
StatePublished - Jul 13 1979

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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