Abstract
High-performance liquid chromatography with microparticulate silica gel and octadecyl silica reversed-phase columns accords ready resolution of the common autoxidation products of cholesterol. Resolution of two pairs of isomeric sterols (5,6α-epoxy-5α-cholestan-3β-ol and 5,6β-epoxy-5β-cholestan-3β-ol, 3β-hydroxy-5α-cholest-6-ene-5-hydroperoxide and 3β-hydroxycholest-5-ene-7α-hydroperoxide) not previously resolved chromatographically has been achieved in both systems. Application of the procedure to the isolation of cholesta-4,6-dien-3-one from autoxidized cholesterol is described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 307-315 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 175 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 13 1979 |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry