High Potency of a Bivalent Human VH Domain in SARS-CoV-2 Animal Models

Wei Li; Alexandra Schäfer; Swarali S. Kulkarni; Xianglei Liu; David R. Martinez; Chuan Chen; Zehua Sun; Sarah R. Leist; Aleksandra Drelich; Liyong Zhang; Marcin L. Ura; Alison Berezuk; Sagar Chittori; Karoline Leopold; Dhiraj Mannar; Shanti S. Srivastava; Xing Zhu; Eric C. Peterson; Chien Te Tseng; John W. Mellors; Darryl Falzarano; Sriram Subramaniam; Ralph S. Baric; Dimiter S. Dimitrov

doi:10.1016/j.cell.2020.09.007

High Potency of a Bivalent Human V_H Domain in SARS-CoV-2 Animal Models

Wei Li
, Alexandra Schäfer
, Swarali S. Kulkarni
, Xianglei Liu
, David R. Martinez
, Chuan Chen
, Zehua Sun
, Sarah R. Leist
, Aleksandra Drelich
, Liyong Zhang
, Marcin L. Ura
, Alison Berezuk
, Sagar Chittori
, Karoline Leopold
, Dhiraj Mannar
, Shanti S. Srivastava
, Xing Zhu
, Eric C. Peterson
, Chien Te Tseng
, John W. Mellors

Darryl Falzarano, Sriram Subramaniam, Ralph S. Baric, Dimiter S. Dimitrov

Microbiology And Immunology

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

A high-affinity human antibody domain, V_H ab8, specific for SARS-CoV-2, bound to all three S protomers competing with ACE2. The relatively small size and bivalency of V_H-Fc ab8 contributed to its high potency in two animal models of infection.

Original language	English (US)
Pages (from-to)	429-441.e16
Journal	Cell
Volume	183
Issue number	2
DOIs	https://doi.org/10.1016/j.cell.2020.09.007
State	Published - Oct 15 2020

Keywords

SARS-CoV-2
electron microscopy
human V antibody domain
mouse and hamster models
virus neutralization

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1016/j.cell.2020.09.007

Cite this

Li, W., Schäfer, A., Kulkarni, S. S., Liu, X., Martinez, D. R., Chen, C., Sun, Z., Leist, S. R., Drelich, A., Zhang, L., Ura, M. L., Berezuk, A., Chittori, S., Leopold, K., Mannar, D., Srivastava, S. S., Zhu, X., Peterson, E. C., Tseng, C. T., ... Dimitrov, D. S. (2020). High Potency of a Bivalent Human V_H Domain in SARS-CoV-2 Animal Models. Cell, 183(2), 429-441.e16. https://doi.org/10.1016/j.cell.2020.09.007

Li, W, Schäfer, A, Kulkarni, SS, Liu, X, Martinez, DR, Chen, C, Sun, Z, Leist, SR, Drelich, A, Zhang, L, Ura, ML, Berezuk, A, Chittori, S, Leopold, K, Mannar, D, Srivastava, SS, Zhu, X, Peterson, EC, Tseng, CT, Mellors, JW, Falzarano, D, Subramaniam, S, Baric, RS & Dimitrov, DS 2020, 'High Potency of a Bivalent Human V_H Domain in SARS-CoV-2 Animal Models', Cell, vol. 183, no. 2, pp. 429-441.e16. https://doi.org/10.1016/j.cell.2020.09.007

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title = "High Potency of a Bivalent Human VH Domain in SARS-CoV-2 Animal Models",

abstract = "A high-affinity human antibody domain, VH ab8, specific for SARS-CoV-2, bound to all three S protomers competing with ACE2. The relatively small size and bivalency of VH-Fc ab8 contributed to its high potency in two animal models of infection.",

keywords = "SARS-CoV-2, electron microscopy, human V antibody domain, mouse and hamster models, virus neutralization",

author = "Wei Li and Alexandra Sch{\"a}fer and Kulkarni, \{Swarali S.\} and Xianglei Liu and Martinez, \{David R.\} and Chuan Chen and Zehua Sun and Leist, \{Sarah R.\} and Aleksandra Drelich and Liyong Zhang and Ura, \{Marcin L.\} and Alison Berezuk and Sagar Chittori and Karoline Leopold and Dhiraj Mannar and Srivastava, \{Shanti S.\} and Xing Zhu and Peterson, \{Eric C.\} and Tseng, \{Chien Te\} and Mellors, \{John W.\} and Darryl Falzarano and Sriram Subramaniam and Baric, \{Ralph S.\} and Dimitrov, \{Dimiter S.\}",

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AU - Li, Wei

AU - Schäfer, Alexandra

AU - Kulkarni, Swarali S.

AU - Liu, Xianglei

AU - Martinez, David R.

AU - Chen, Chuan

AU - Sun, Zehua

AU - Leist, Sarah R.

AU - Drelich, Aleksandra

AU - Zhang, Liyong

AU - Ura, Marcin L.

AU - Berezuk, Alison

AU - Chittori, Sagar

AU - Leopold, Karoline

AU - Mannar, Dhiraj

AU - Srivastava, Shanti S.

AU - Zhu, Xing

AU - Peterson, Eric C.

AU - Tseng, Chien Te

AU - Mellors, John W.

AU - Falzarano, Darryl

AU - Subramaniam, Sriram

AU - Baric, Ralph S.

AU - Dimitrov, Dimiter S.

PY - 2020/10/15

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