TY - JOUR
T1 - High pressure liquid chromatographic separation of two major toxic compounds from gymnodinium Breve Davis
AU - Risk, Martin
AU - Lin, Y. Y.
AU - Sadagopa Ramanujam, V. M.
AU - Smith, Leland L.
AU - Ray, Sammy M.
AU - Trieff, Norman M.
N1 - Funding Information:
We wish to express appreciation to Ms. A. Aldrich for culturing of G. breve, to Dr. G. Ansari for technical suggestions, to Darryl Friday of Waters Associates for the loan of the semi-preparative HPLC column and RI detector. This research was supported in part by Grant H-416 from Robert A. Welch Foundation, Texas, and the Donald A. Rappoport Memorial Fund, UTMB, Galveston, Texas.
PY - 1979/7
Y1 - 1979/7
N2 - Cultured Gymnodinium breve cells were extracted in acidified ether and fractionated by a new, convenient procedure utilizing thin layer chromatography or elution dry column chromatography. The most toxic fraction was further separated either directly by analytical high pressure liquid chromatography (HPLC) or in subsequent work by preparative, followed by analytical HPLC. Two toxic compounds, designated T4g and T47, were isolated; purity of each was demonstrated by rechromatography in analytical HPLC with both adsorptive and reverse phase packings. Both the single pass and recycle modes of operation were used with two detector systems in each to demonstrate a single entity. UV, fluorescence, and stability data differentiated these compounds from previously described, less toxic components from 6. breve.
AB - Cultured Gymnodinium breve cells were extracted in acidified ether and fractionated by a new, convenient procedure utilizing thin layer chromatography or elution dry column chromatography. The most toxic fraction was further separated either directly by analytical high pressure liquid chromatography (HPLC) or in subsequent work by preparative, followed by analytical HPLC. Two toxic compounds, designated T4g and T47, were isolated; purity of each was demonstrated by rechromatography in analytical HPLC with both adsorptive and reverse phase packings. Both the single pass and recycle modes of operation were used with two detector systems in each to demonstrate a single entity. UV, fluorescence, and stability data differentiated these compounds from previously described, less toxic components from 6. breve.
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U2 - 10.1093/chromsci/17.7.400
DO - 10.1093/chromsci/17.7.400
M3 - Article
C2 - 573279
AN - SCOPUS:0018584821
SN - 0021-9665
VL - 17
SP - 400
EP - 405
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 7
ER -