High pressure liquid chromatographic separation of two major toxic compounds from gymnodinium Breve Davis

Martin Risk, Y. Y. Lin, V. M. Sadagopa Ramanujam, Leland L. Smith, Sammy M. Ray, Norman M. Trieff

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Cultured Gymnodinium breve cells were extracted in acidified ether and fractionated by a new, convenient procedure utilizing thin layer chromatography or elution dry column chromatography. The most toxic fraction was further separated either directly by analytical high pressure liquid chromatography (HPLC) or in subsequent work by preparative, followed by analytical HPLC. Two toxic compounds, designated T4g and T47, were isolated; purity of each was demonstrated by rechromatography in analytical HPLC with both adsorptive and reverse phase packings. Both the single pass and recycle modes of operation were used with two detector systems in each to demonstrate a single entity. UV, fluorescence, and stability data differentiated these compounds from previously described, less toxic components from 6. breve.

Original languageEnglish (US)
Pages (from-to)400-405
Number of pages6
JournalJournal of Chromatographic Science
Volume17
Issue number7
DOIs
StatePublished - Jul 1979

ASJC Scopus subject areas

  • Analytical Chemistry

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