High protein diets are associated with increased bacterial translocation in septic guinea pigs

Jeffrey L. Nelson, J. W. Alexander, L. Gianotti, C. L. Chalk, Tonyia Eaves-Pyles

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

During sepsis, body protein stores are decreased due to an increase in protein catabolism. The utilization of nutritional support with high-protein diets has been used as a solution to the problem of sepsis-induced protein loss. Work from our laboratory, however, has shown that diets low in protein (5% of total calories) improve survival in septic animals as compared to high protein (20%) diets. The present study investigated the relationship between low protein diets and improved survival by determining whether septic animals receiving high-protein diets have increased bacterial translocation. Sepsis was induced in guinea pigs by the implantation of an osmotic minipump into the peritoneal cavity containing an equal mixture of Escherichia coli (108) and Staphylococcus aureus (108) or saline. On Day 3 postlaparotomy, the animals were randomized to one of four groups. The groups consisted of septic and nonseptic animals that received a diet with 5 or 20% of total calories as protein. Following 4 days of diet all animals received an instillation of 14C labeled E. coli (1010). Four hours later the animals were sacrificed and blood, mesenteric lymph nodes, spleen, lungs, and liver were removed for determination of radionuclide counts. Results indicated that the septic animals that received the high protein diet had more bacterial translocation, as indexed by higher radionuclide counts in the MLN, liver, lung and blood. These findings suggest that a low protein, enterally fed diet may improve survival in septic patients by decreasing the incidence of bacterial translocation.

Original languageEnglish (US)
Pages (from-to)195-199
Number of pages5
JournalNutrition
Volume12
Issue number3
DOIs
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Bacterial Translocation
Guinea Pigs
Diet
Proteins
Sepsis
Protein-Restricted Diet
Radioisotopes
Survival
Escherichia coli
Lung
Nutritional Support
Liver
Peritoneal Cavity
Staphylococcus aureus
Spleen
Lymph Nodes

Keywords

  • bacterial translocation
  • enteral nutrition
  • Escherichia coli
  • guinea pig
  • intestinal function
  • protein
  • sepsis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Surgery
  • Medicine (miscellaneous)

Cite this

High protein diets are associated with increased bacterial translocation in septic guinea pigs. / Nelson, Jeffrey L.; Alexander, J. W.; Gianotti, L.; Chalk, C. L.; Eaves-Pyles, Tonyia.

In: Nutrition, Vol. 12, No. 3, 03.1996, p. 195-199.

Research output: Contribution to journalArticle

Nelson, Jeffrey L. ; Alexander, J. W. ; Gianotti, L. ; Chalk, C. L. ; Eaves-Pyles, Tonyia. / High protein diets are associated with increased bacterial translocation in septic guinea pigs. In: Nutrition. 1996 ; Vol. 12, No. 3. pp. 195-199.
@article{8fe905ccd31b4c398428d1a3a9db2256,
title = "High protein diets are associated with increased bacterial translocation in septic guinea pigs",
abstract = "During sepsis, body protein stores are decreased due to an increase in protein catabolism. The utilization of nutritional support with high-protein diets has been used as a solution to the problem of sepsis-induced protein loss. Work from our laboratory, however, has shown that diets low in protein (5{\%} of total calories) improve survival in septic animals as compared to high protein (20{\%}) diets. The present study investigated the relationship between low protein diets and improved survival by determining whether septic animals receiving high-protein diets have increased bacterial translocation. Sepsis was induced in guinea pigs by the implantation of an osmotic minipump into the peritoneal cavity containing an equal mixture of Escherichia coli (108) and Staphylococcus aureus (108) or saline. On Day 3 postlaparotomy, the animals were randomized to one of four groups. The groups consisted of septic and nonseptic animals that received a diet with 5 or 20{\%} of total calories as protein. Following 4 days of diet all animals received an instillation of 14C labeled E. coli (1010). Four hours later the animals were sacrificed and blood, mesenteric lymph nodes, spleen, lungs, and liver were removed for determination of radionuclide counts. Results indicated that the septic animals that received the high protein diet had more bacterial translocation, as indexed by higher radionuclide counts in the MLN, liver, lung and blood. These findings suggest that a low protein, enterally fed diet may improve survival in septic patients by decreasing the incidence of bacterial translocation.",
keywords = "bacterial translocation, enteral nutrition, Escherichia coli, guinea pig, intestinal function, protein, sepsis",
author = "Nelson, {Jeffrey L.} and Alexander, {J. W.} and L. Gianotti and Chalk, {C. L.} and Tonyia Eaves-Pyles",
year = "1996",
month = "3",
doi = "10.1016/S0899-9007(96)00051-2",
language = "English (US)",
volume = "12",
pages = "195--199",
journal = "Nutrition International",
issn = "0899-9007",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - High protein diets are associated with increased bacterial translocation in septic guinea pigs

AU - Nelson, Jeffrey L.

AU - Alexander, J. W.

AU - Gianotti, L.

AU - Chalk, C. L.

AU - Eaves-Pyles, Tonyia

PY - 1996/3

Y1 - 1996/3

N2 - During sepsis, body protein stores are decreased due to an increase in protein catabolism. The utilization of nutritional support with high-protein diets has been used as a solution to the problem of sepsis-induced protein loss. Work from our laboratory, however, has shown that diets low in protein (5% of total calories) improve survival in septic animals as compared to high protein (20%) diets. The present study investigated the relationship between low protein diets and improved survival by determining whether septic animals receiving high-protein diets have increased bacterial translocation. Sepsis was induced in guinea pigs by the implantation of an osmotic minipump into the peritoneal cavity containing an equal mixture of Escherichia coli (108) and Staphylococcus aureus (108) or saline. On Day 3 postlaparotomy, the animals were randomized to one of four groups. The groups consisted of septic and nonseptic animals that received a diet with 5 or 20% of total calories as protein. Following 4 days of diet all animals received an instillation of 14C labeled E. coli (1010). Four hours later the animals were sacrificed and blood, mesenteric lymph nodes, spleen, lungs, and liver were removed for determination of radionuclide counts. Results indicated that the septic animals that received the high protein diet had more bacterial translocation, as indexed by higher radionuclide counts in the MLN, liver, lung and blood. These findings suggest that a low protein, enterally fed diet may improve survival in septic patients by decreasing the incidence of bacterial translocation.

AB - During sepsis, body protein stores are decreased due to an increase in protein catabolism. The utilization of nutritional support with high-protein diets has been used as a solution to the problem of sepsis-induced protein loss. Work from our laboratory, however, has shown that diets low in protein (5% of total calories) improve survival in septic animals as compared to high protein (20%) diets. The present study investigated the relationship between low protein diets and improved survival by determining whether septic animals receiving high-protein diets have increased bacterial translocation. Sepsis was induced in guinea pigs by the implantation of an osmotic minipump into the peritoneal cavity containing an equal mixture of Escherichia coli (108) and Staphylococcus aureus (108) or saline. On Day 3 postlaparotomy, the animals were randomized to one of four groups. The groups consisted of septic and nonseptic animals that received a diet with 5 or 20% of total calories as protein. Following 4 days of diet all animals received an instillation of 14C labeled E. coli (1010). Four hours later the animals were sacrificed and blood, mesenteric lymph nodes, spleen, lungs, and liver were removed for determination of radionuclide counts. Results indicated that the septic animals that received the high protein diet had more bacterial translocation, as indexed by higher radionuclide counts in the MLN, liver, lung and blood. These findings suggest that a low protein, enterally fed diet may improve survival in septic patients by decreasing the incidence of bacterial translocation.

KW - bacterial translocation

KW - enteral nutrition

KW - Escherichia coli

KW - guinea pig

KW - intestinal function

KW - protein

KW - sepsis

UR - http://www.scopus.com/inward/record.url?scp=0030110911&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030110911&partnerID=8YFLogxK

U2 - 10.1016/S0899-9007(96)00051-2

DO - 10.1016/S0899-9007(96)00051-2

M3 - Article

C2 - 8798224

AN - SCOPUS:0030110911

VL - 12

SP - 195

EP - 199

JO - Nutrition International

JF - Nutrition International

SN - 0899-9007

IS - 3

ER -