Abstract
Electron cryomicroscopy is a high-resolution imaging technique that is particularly appropriate for the structural determination of large macromolecular assemblies, biological molecules that form two-dimensional crystals, the application of electron cryomicroscopy and image reconstruction can help elucidate structures at atomic resolution. In instances where crystals cannot be formed, atomic-resolution information can be obtained by combining high-resolution structures of individual components determined by X-ray crystallography or NMR with image-derived reconstructions at moderate resolution. This can provide unique and crucial information on the mechanisms of these complexes. Finally, image reconstructions can be used to augment X- ray studies by providing initial models that facilitate phasing of crystals of large macromolecular machines such as ribosomes and viruses.
Original language | English (US) |
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Pages (from-to) | 154-159 |
Number of pages | 6 |
Journal | Trends in Cell Biology |
Volume | 9 |
Issue number | 4 |
DOIs | |
State | Published - Apr 1 1999 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology