High-resolution experimental phases for tryptophanyl-tRNA synthetase (TrpRS) complexed with tryptophanyl-5′AMP

P. Retailleau, Y. Yin, M. Hu, J. Roach, G. Bricogne, C. Vonrhein, P. Roversi, E. Blanc, R. M. Sweet, Jr Carter

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Native data, anomalous data at three wavelengths and an independent peak-wavelength data set for SeMet-substituted protein have been collected from cryoprotected crystals of the TrpRS-adenylate product (TAM) complex to a resolution limit of 1.7 Å. Independent phase sets were developed using SHARP and improved by solvent flipping with SOLOMON using molecular envelopes derived from experimental densities for, respectively, peak-wavelength SAD data from four different crystals, MAD data and their M(S)IRAS combinations with native data. Hendrickson-Lattman phase-probability coefficients from each phase set were used in BUSTER to drive maximum-likelihood refinements of well defined parts of the previously refined room-temperature 2.9 Å structure. Maximum-entropy completion followed by manual rebuilding was then used to generate a model for the missing segments, bound ligand and solvent molecules. Surprisingly, peak-wavelength SAD experiments produced the smallest phase errors relative to the refined structures. Selenomethionylated models deviate from one another by 0.25 Å and from the native model by 0.38 Å, but all have r.m.s. deviations of ∼1.0 Å from the 2.9 Å model. Difference Fourier calculations between amplitudes from the 300 K experiment and the new amplitudes at 100 K using 1.7 model phases show no significant structural changes arising from temperature variation or addition of cryoprotectant. The main differences between low- and high-resolution structures arise from correcting side-chain rotamers in the core of the protein as well as on the surface. These changes improve various structure-validation criteria.

Original languageEnglish (US)
Pages (from-to)1595-1608
Number of pages14
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number11
DOIs
StatePublished - Nov 20 2001
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology

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