High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals

David G. Gorenstein, Evelyn M. Goldfield, Roulhwai Chen, Ken Kovar, Bruce A. Luxon

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal ion experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P. J. M., Swarthof, T., & Hilbers, C. W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.

Original languageEnglish (US)
Pages (from-to)2141-2150
Number of pages10
JournalBiochemistry
Volume20
Issue number8
StatePublished - 1981
Externally publishedYes

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Transfer RNA
Phenylalanine
Yeast
Phosphorus
Metal ions
Magnetic Resonance Spectroscopy
Yeasts
Metals
Nuclear magnetic resonance
Ions
Biochemistry
Magnesium
Experiments
Phosphates
Binding Sites
X-Rays
X rays
Molecules
Temperature
Monitoring

ASJC Scopus subject areas

  • Biochemistry

Cite this

High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals. / Gorenstein, David G.; Goldfield, Evelyn M.; Chen, Roulhwai; Kovar, Ken; Luxon, Bruce A.

In: Biochemistry, Vol. 20, No. 8, 1981, p. 2141-2150.

Research output: Contribution to journalArticle

Gorenstein, David G. ; Goldfield, Evelyn M. ; Chen, Roulhwai ; Kovar, Ken ; Luxon, Bruce A. / High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals. In: Biochemistry. 1981 ; Vol. 20, No. 8. pp. 2141-2150.
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AU - Luxon, Bruce A.

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