Abstract
The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal ion experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P. J. M., Swarthof, T., & Hilbers, C. W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2141-2150 |
| Number of pages | 10 |
| Journal | Biochemistry |
| Volume | 20 |
| Issue number | 8 |
| State | Published - 1981 |
| Externally published | Yes |
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- Biochemistry
Cite this
High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals. / Gorenstein, David G.; Goldfield, Evelyn M.; Chen, Roulhwai; Kovar, Ken; Luxon, Bruce A.
In: Biochemistry, Vol. 20, No. 8, 1981, p. 2141-2150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-resolution phosphorus nuclear magnetic resonance spectra of yeast phenylalanine transfer ribonucleic acid. Metal ion effects and tentative partial assignment of signals
AU - Gorenstein, David G.
AU - Goldfield, Evelyn M.
AU - Chen, Roulhwai
AU - Kovar, Ken
AU - Luxon, Bruce A.
PY - 1981
Y1 - 1981
N2 - The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal ion experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P. J. M., Swarthof, T., & Hilbers, C. W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.
AB - The temperature and metal ion dependence of the 31P NMR spectra of yeast phenylalanine tRNA is presented, and a tentative assignment of some of the individual phosphate signals is offered. Signals A, C, F, P, and T have been assigned to the magnesium binding site phosphates by monitoring the magnesium ion dependence in the 31P NMR spectra. In the presence of 10 mM Mg2+, specific paramagnetic line broadening effects are observed for signals A, D, E, and U upon addition of 0.001-0.1 Mn2+ ion per tRNA molecule. Through the combination of the above metal ion experiments, X-ray studies, and earlier 1H and 31P NMR studies [particularly the tRNA modification 31P NMR experiments of Salemink et al. [Salemink, P. J. M., Swarthof, T., & Hilbers, C. W. (1979) Biochemistry 18, 3477]], an initial assignment of some of these signals is attempted.
UR - http://www.scopus.com/inward/record.url?scp=0019879666&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019879666&partnerID=8YFLogxK
M3 - Article
VL - 20
SP - 2141
EP - 2150
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 8
ER -