High-resolution timing of cell cycle-regulated gene expression

Maga Rowicka, Andrzej Kudlicki, Benjamin P. Tu, Zbyszek Otwinowski

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


The eukaryotic cell division cycle depends on an intricate sequence of transcriptional events. Using an algorithm based on maximum-entropy deconvolution, and expression data from a highly synchronized yeast culture, we have timed the peaks of expression of transcriptionally regulated cell cycle genes to an accuracy of 2 min (≈1% of the cell cycle time). The set of 1,129 cell cycle-regulated genes was identified by a comprehensive analysis encompassing all available cell cycle yeast data sets. Our results reveal distinct subphases of the cell cycle undetectable by morphological observation, as well as the precise timeline of macromolecular complex assembly during key cell cycle events.

Original languageEnglish (US)
Pages (from-to)16892-16897
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number43
StatePublished - Oct 23 2007


  • Maximum entropy
  • Microarrays
  • Mitosis
  • Yeast

ASJC Scopus subject areas

  • General


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