High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion

Zee Yong Park, Rovshan Sadygov, John R. Yates

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of computer algorithm for analyzing the cleavage pattern by mass spectrometry to map disulfide bonds was discussed. This labeling method had shown to be effective especially in mapping complicated disulfide bond patterns of adjacent cysteins. In the latest disulfide bond mapping method minimum sample preparation and computer based data analysis were used. Results show that three disulfide bonds of lysozyme and ten disulfide bonds of bovine serum albumin were easily mapped by the method without human intervention.

Original languageEnglish (US)
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages43-44
Number of pages2
StatePublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Disulfides
Throughput
Proteins
Muramidase
Bovine Serum Albumin
Labeling
Mass spectrometry

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Park, Z. Y., Sadygov, R., & Yates, J. R. (2002). High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 43-44)

High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. / Park, Zee Yong; Sadygov, Rovshan; Yates, John R.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 43-44.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Park, ZY, Sadygov, R & Yates, JR 2002, High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 43-44, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Park ZY, Sadygov R, Yates JR. High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 43-44
Park, Zee Yong ; Sadygov, Rovshan ; Yates, John R. / High throughput mapping of disulfide bonds in proteins by a computer algorithm combining MS/MS and accurate mass data from enzymatic digestion. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 43-44
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