High-throughput method for on-target performic acid oxidation of MALDI-deposited samples

Brad J. Williams, William K. Russell, David H. Russell

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

An information-rich on-target performic acid oxidation method, which is compatible with alkylation for differentiation of free cysteine versus disulfide-containing peptides, is described. On-target oxidation is achieved using performic acid vapor to oxidize disulfide-containing peptides and/or small proteins on the matrix-assisted laser desorption/ionization (MALDI) sample deposits. The on-target oxidation method is preferred over solution-phase oxidation methods because (1) less sample handing is required, (2) oxidation throughput is drastically increased and (3) ion suppression effects are reduced because performic acid is not added directly to the MALDI spot. The utility of this method is demonstrated by simultaneous oxidation of multiple MALDI sample deposits containing model disulfide-linked peptides, intact bovine insulin and a bovine ribonuclease A proteolytic digest.

Original languageEnglish (US)
Pages (from-to)157-166
Number of pages10
JournalJournal of Mass Spectrometry
Volume45
Issue number2
DOIs
StatePublished - Feb 2010

Keywords

  • Disulfide bonds
  • MALDI-MS
  • On-target oxidation
  • Performic acid vapor
  • Ribonuclease A

ASJC Scopus subject areas

  • Spectroscopy

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