High-throughput multistrain polymerase chain reaction quantification of Chlamydia trachomatis from clinical and preclinical urogenital specimens

Chris L. McGowin, Gregory C. Whitlock, Richard Pyles

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chlamydia trachomatis (CT) is the most prevalent sexually transmitted bacterial pathogen worldwide and causes severe reproductive tract infections. Currently, nucleic acid amplification tests (NAATs) are the gold standard for clinical diagnosis, but most NAATs are labor intensive and limited to specific CT serovars. We developed and validated a quantitative polymerase chain reaction (qPCR) assay that reproducibly detected CT serovars D, E, F, Ia, and Chlamydia muridarum over a linear range of 2 log10 to 10 log10 genomes with low coefficients of variation from both experimental and human urine samples. CT DNA loads from human vaginal, endocervical, and male urethral swabs correlated well with the BD ProbeTec ET assay (Becton Dickinson Diagnostic Systems, Franklin Lakes, NJ) run in parallel. In a preclinical microbicide evaluation, C. muridarum DNA loads in mouse swabs and tissues correlated well with an immunofluorescence assay. The optimized qPCR system provided enhanced sensitivity and facilitated the quantitative evaluation of clinical and experimental preclinical samples for anti-CT therapeutic and microbicide evaluation.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume64
Issue number2
DOIs
StatePublished - Jun 2009

Fingerprint

Chlamydia trachomatis
Chlamydia muridarum
Polymerase Chain Reaction
Nucleic Acid Amplification Techniques
Anti-Infective Agents
Reproductive Tract Infections
DNA
Lakes
Fluorescent Antibody Technique
Urine
Genome
Serogroup

Keywords

  • Chlamydia muridarum
  • Chlamydia trachomatis
  • Diagnostic
  • Real-time PCR
  • Sexually transmitted infection

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

High-throughput multistrain polymerase chain reaction quantification of Chlamydia trachomatis from clinical and preclinical urogenital specimens. / McGowin, Chris L.; Whitlock, Gregory C.; Pyles, Richard.

In: Diagnostic Microbiology and Infectious Disease, Vol. 64, No. 2, 06.2009, p. 117-123.

Research output: Contribution to journalArticle

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