Histamine-releasing activity (HRA). III. HRA induces human basophil histamine release by provoking noncytotoxic granule exocytosis

Ann M. Dvorak, Michael A. Lett-Brown, David O. Thueson, Kathryn Pyne, Puthalath K. Raghuprasad, Stephen J. Galli, J. Andrew Grant

Research output: Contribution to journalArticle

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Abstract

Histamine-releasing activity (HRA) is an ∼10,000-15,000 dalton, protease-sensitive factor that induces the rapid liberation of histamine from human basophils. Production of HRA by human peripheral blood mononuclear cells in vitro is augmented by concanavalin A or antigen, suggesting a mechanism whereby lymphocytes may regulate basophil mediator release in vivo. In order to determine whether HRA provokes conventional exocytosis of basophil granules or, alternatively, results in mediator release by some other mechanism such as vesicular transport or cytotoxicity, we investigated the ultrastructural features of human blood basophils purified over Percoll and exposed to HRA in vitro. HRA preparations induced a noncytotoxic pattern of basophil degranelation very similar to that previously observed in basophils triggered to release histamine in response to specific antigen, C5a, or mannitol. Thus, cytoplasmic granules were extruded singly hrough multiple separate points of fusion between perigranular membranes and the plasma membrane. Degranulating basophils exhibited plasma membrane activation but lacked a polarized configuration. By contrast, those basophils exposed to HRA that did not exhibit evidence of degranulation displayed a single elongated cellular process. The development of this polarized cellular configuration, similar in some respects to that of uropod-bearing motile guinea pig basophils, may have reflected chemokinetic or chemotactic effects of preparations containing HRA activity.

Original languageEnglish (US)
Pages (from-to)142-150
Number of pages9
JournalClinical Immunology and Immunopathology
Volume32
Issue number2
DOIs
StatePublished - 1984

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Basophils
Histamine Release
Exocytosis
Human Activities
Histamine
Cell Membrane
Cytoplasmic Granules
Antigens
Mannitol
Concanavalin A
Blood Cells
Guinea Pigs
Peptide Hydrolases
Lymphocytes
Membranes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Histamine-releasing activity (HRA). III. HRA induces human basophil histamine release by provoking noncytotoxic granule exocytosis. / Dvorak, Ann M.; Lett-Brown, Michael A.; Thueson, David O.; Pyne, Kathryn; Raghuprasad, Puthalath K.; Galli, Stephen J.; Grant, J. Andrew.

In: Clinical Immunology and Immunopathology, Vol. 32, No. 2, 1984, p. 142-150.

Research output: Contribution to journalArticle

Dvorak, Ann M. ; Lett-Brown, Michael A. ; Thueson, David O. ; Pyne, Kathryn ; Raghuprasad, Puthalath K. ; Galli, Stephen J. ; Grant, J. Andrew. / Histamine-releasing activity (HRA). III. HRA induces human basophil histamine release by provoking noncytotoxic granule exocytosis. In: Clinical Immunology and Immunopathology. 1984 ; Vol. 32, No. 2. pp. 142-150.
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