TY - JOUR
T1 - Histamine releasing activity (HRA) produced by leukocytes co-cultured with tumor cells
AU - Langford, Marlyn P.
AU - Lett-Brown, Micheal A.
AU - Stanton, G. John
AU - Grant, J. Andrew
N1 - Funding Information:
ACKNOWLEDGEMENTS The authors thank Yvonne Hillier and Lisa Robinson for excellent technical assistance. These studies were supported in part by PHS Grant EY017015, NIH Research Award AI-12621 and Cancer Core Grant CA-17701.
PY - 1987
Y1 - 1987
N2 - Human stomach adenocarcinoma (AGS), astrocytoma (AST) and mylogenous leukemia (K562) cells were co-cultured with human peripheral blood leukocytes (PBL) through four days. Histamine releasing activity (HRA, lymphokines that stimulate degranulation of basophilic leukocytes with the release of histamine) and interferons alpha and gamma (IFNα and IFNγ were detected in the culture fluids. Maximal levels of HRA were detected by 24 hr in AST and K562-leukocyte co-culture fluids. Notably, only low levels of HRA was detected in AGS-leukocyte cultures. HRA was separated into two molecular weight species (60,000 and 25,000) by column chromatography. The IFN activity was shown to be a mixture of IFNα and IFNγ (IFNαgt;IFNγ IFN did not cause histamine release from human PEL or affect HRA. Our results indicate that PBL from normal individuals produce HRA in response to tumor antigen and suggest that the basophilic leukocyte response to certain cancers may be related to HRA production.
AB - Human stomach adenocarcinoma (AGS), astrocytoma (AST) and mylogenous leukemia (K562) cells were co-cultured with human peripheral blood leukocytes (PBL) through four days. Histamine releasing activity (HRA, lymphokines that stimulate degranulation of basophilic leukocytes with the release of histamine) and interferons alpha and gamma (IFNα and IFNγ were detected in the culture fluids. Maximal levels of HRA were detected by 24 hr in AST and K562-leukocyte co-culture fluids. Notably, only low levels of HRA was detected in AGS-leukocyte cultures. HRA was separated into two molecular weight species (60,000 and 25,000) by column chromatography. The IFN activity was shown to be a mixture of IFNα and IFNγ (IFNαgt;IFNγ IFN did not cause histamine release from human PEL or affect HRA. Our results indicate that PBL from normal individuals produce HRA in response to tumor antigen and suggest that the basophilic leukocyte response to certain cancers may be related to HRA production.
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U2 - 10.3109/08820138709030570
DO - 10.3109/08820138709030570
M3 - Article
C2 - 2442099
AN - SCOPUS:0023238123
SN - 0882-0139
VL - 16
SP - 129
EP - 138
JO - Immunological Investigations
JF - Immunological Investigations
IS - 2
ER -