Histochemical alterations of acute and chronic doxorubicin cardiotoxicity

Renee C. Aversano, Paul J. Boor

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35 Scopus citations

Abstract

Histochemical alterations of acute and chronic doxorubicin (DOX) cardiotoxicity in the mouse were assessed by the localization of succinate dehydrogenase (SDH), coenzyme Q10 (CoQ), cytochrome oxidase (COX), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), reduced glutathione (GSH), and intracellular calcium. Isolated myocytes intensely stained for calcium were found at 72 and 120 h under the acute protocol; altered staining patterns of SDH, CoQ, and COX, were evident at 120 h. Chronically, two patterns of intracellular calcium staining were evident: (1) intensely stained myocytes as found in the acute protocol; and (2) multiple discrete intracellular deposits suggestive of mitochondrial localization. Altered staining patterns of SDH, CoQ, COX, CPK, and LDH under the chronic protocol were only seen after abnormal staining was evident in trichrome stained sections. The presence of characteristic vacuolated myocardial cells in both acute and chronic protocols was confirmed by one micron epon-embedded toluidine blue stained sections and electron microscopy. These histochemical findings suggest that DOX alters the functional integrity of mitochondrial respiratory chain enzymes in the myocardial cell.

Original languageEnglish (US)
Pages (from-to)543-553
Number of pages11
JournalJournal of Molecular and Cellular Cardiology
Volume15
Issue number8
DOIs
StatePublished - 1983

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Keywords

  • Cardiomyopathy
  • Coenzyme Q
  • Cytochrome Oxidase
  • Doxorubicin
  • Histochemistry
  • Intracellular calcium
  • Mitochondria
  • Succinate dehydrogenase

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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