Abstract
Histochemical alterations of acute and chronic doxorubicin (DOX) cardiotoxicity in the mouse were assessed by the localization of succinate dehydrogenase (SDH), coenzyme Q10 (CoQ), cytochrome oxidase (COX), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), reduced glutathione (GSH), and intracellular calcium. Isolated myocytes intensely stained for calcium were found at 72 and 120 h under the acute protocol; altered staining patterns of SDH, CoQ, and COX, were evident at 120 h. Chronically, two patterns of intracellular calcium staining were evident: (1) intensely stained myocytes as found in the acute protocol; and (2) multiple discrete intracellular deposits suggestive of mitochondrial localization. Altered staining patterns of SDH, CoQ, COX, CPK, and LDH under the chronic protocol were only seen after abnormal staining was evident in trichrome stained sections. The presence of characteristic vacuolated myocardial cells in both acute and chronic protocols was confirmed by one micron epon-embedded toluidine blue stained sections and electron microscopy. These histochemical findings suggest that DOX alters the functional integrity of mitochondrial respiratory chain enzymes in the myocardial cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 543-553 |
| Number of pages | 11 |
| Journal | Journal of Molecular and Cellular Cardiology |
| Volume | 15 |
| Issue number | 8 |
| DOIs | |
| State | Published - 1983 |
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Keywords
- Cardiomyopathy
- Coenzyme Q
- Cytochrome Oxidase
- Doxorubicin
- Histochemistry
- Intracellular calcium
- Mitochondria
- Succinate dehydrogenase
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine
Cite this
Histochemical alterations of acute and chronic doxorubicin cardiotoxicity. / Aversano, Renee C.; Boor, Paul J.
In: Journal of Molecular and Cellular Cardiology, Vol. 15, No. 8, 1983, p. 543-553.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Histochemical alterations of acute and chronic doxorubicin cardiotoxicity
AU - Aversano, Renee C.
AU - Boor, Paul J.
PY - 1983
Y1 - 1983
N2 - Histochemical alterations of acute and chronic doxorubicin (DOX) cardiotoxicity in the mouse were assessed by the localization of succinate dehydrogenase (SDH), coenzyme Q10 (CoQ), cytochrome oxidase (COX), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), reduced glutathione (GSH), and intracellular calcium. Isolated myocytes intensely stained for calcium were found at 72 and 120 h under the acute protocol; altered staining patterns of SDH, CoQ, and COX, were evident at 120 h. Chronically, two patterns of intracellular calcium staining were evident: (1) intensely stained myocytes as found in the acute protocol; and (2) multiple discrete intracellular deposits suggestive of mitochondrial localization. Altered staining patterns of SDH, CoQ, COX, CPK, and LDH under the chronic protocol were only seen after abnormal staining was evident in trichrome stained sections. The presence of characteristic vacuolated myocardial cells in both acute and chronic protocols was confirmed by one micron epon-embedded toluidine blue stained sections and electron microscopy. These histochemical findings suggest that DOX alters the functional integrity of mitochondrial respiratory chain enzymes in the myocardial cell.
AB - Histochemical alterations of acute and chronic doxorubicin (DOX) cardiotoxicity in the mouse were assessed by the localization of succinate dehydrogenase (SDH), coenzyme Q10 (CoQ), cytochrome oxidase (COX), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), reduced glutathione (GSH), and intracellular calcium. Isolated myocytes intensely stained for calcium were found at 72 and 120 h under the acute protocol; altered staining patterns of SDH, CoQ, and COX, were evident at 120 h. Chronically, two patterns of intracellular calcium staining were evident: (1) intensely stained myocytes as found in the acute protocol; and (2) multiple discrete intracellular deposits suggestive of mitochondrial localization. Altered staining patterns of SDH, CoQ, COX, CPK, and LDH under the chronic protocol were only seen after abnormal staining was evident in trichrome stained sections. The presence of characteristic vacuolated myocardial cells in both acute and chronic protocols was confirmed by one micron epon-embedded toluidine blue stained sections and electron microscopy. These histochemical findings suggest that DOX alters the functional integrity of mitochondrial respiratory chain enzymes in the myocardial cell.
KW - Cardiomyopathy
KW - Coenzyme Q
KW - Cytochrome Oxidase
KW - Doxorubicin
KW - Histochemistry
KW - Intracellular calcium
KW - Mitochondria
KW - Succinate dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=0020561739&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020561739&partnerID=8YFLogxK
U2 - 10.1016/0022-2828(83)90330-9
DO - 10.1016/0022-2828(83)90330-9
M3 - Article
C2 - 6672210
AN - SCOPUS:0020561739
VL - 15
SP - 543
EP - 553
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 8
ER -