Histologic chorioamnionitis does not modulate the oxidative stress and antioxidant status in pregnancies complicated by spontaneous preterm delivery

Laura Fernandes Martin, Natália Prearo Moço, Moisés Diôgo de Lima, Jossimara Polettini, Hélio Amante Miot, Camila Renata Corrêa, Ramkumar Menon, Márcia Guimarães da Silva

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Infection induced-inflammation and other risk factors for spontaneous preterm birth (PTB) and preterm premature rupture of membranes (pPROM) may cause a redox imbalance, increasing the release of free radicals and consuming antioxidant defenses. Oxidative stress, in turn, can initiate intracellular signaling cascades that increase the production of pro-inflammatory mediators. The objective of this study was to evaluate the oxidative damage to proteins and antioxidant capacity profiles in amniochorion membranes from preterm birth (PTB) and preterm premature rupture of membranes (pPROM) and to determine the role of histologic chorioamnionitis in this scenario. Methods: We included 27 pregnant women with PTB, 27 pPROM and 30 at term. Protein oxidative damage was assayed by 3-nitrotyrosine (3-NT) and carbonyl levels, using enzyme-linked immunosorbent assay (ELISA) and modified dinitrophenylhydrazine assay (DNPH), respectively. Total antioxidant capacity (TAC) was measured by ELISA. Results: Protein oxidative damage determined by carbonyl levels was lower in PTB group than pPROM and term groups (p < 0.001). PTB group presented higher TAC compared with pPROM and term groups (p = 0.002). Histologic chorioamnionitis did not change either protein oxidative damage or TAC regardless of gestational outcome. Conclusion: These results corroborates previous reports that pPROM and term birth exhibit similarities in oxidative stress- induced senescence and histologic chorioamnionitis does not modulate oxidative stress or antioxidant status.

Original languageEnglish (US)
Article number376
JournalBMC Pregnancy and Childbirth
Volume17
Issue number1
DOIs
StatePublished - Nov 13 2017

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Chorioamnionitis
Premature Birth
Oxidative Stress
Antioxidants
Pregnancy
Proteins
Enzyme-Linked Immunosorbent Assay
Term Birth
Oxidation-Reduction
Free Radicals
Preterm Premature Rupture of the Membranes
Pregnant Women
Inflammation
Membranes
Infection

Keywords

  • Antioxidant capacity
  • Histologic chorioamnionitis
  • Oxidative stress
  • Preterm birth

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Histologic chorioamnionitis does not modulate the oxidative stress and antioxidant status in pregnancies complicated by spontaneous preterm delivery. / Martin, Laura Fernandes; Moço, Natália Prearo; de Lima, Moisés Diôgo; Polettini, Jossimara; Miot, Hélio Amante; Corrêa, Camila Renata; Menon, Ramkumar; da Silva, Márcia Guimarães.

In: BMC Pregnancy and Childbirth, Vol. 17, No. 1, 376, 13.11.2017.

Research output: Contribution to journalArticle

Martin, Laura Fernandes ; Moço, Natália Prearo ; de Lima, Moisés Diôgo ; Polettini, Jossimara ; Miot, Hélio Amante ; Corrêa, Camila Renata ; Menon, Ramkumar ; da Silva, Márcia Guimarães. / Histologic chorioamnionitis does not modulate the oxidative stress and antioxidant status in pregnancies complicated by spontaneous preterm delivery. In: BMC Pregnancy and Childbirth. 2017 ; Vol. 17, No. 1.
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AU - Martin, Laura Fernandes

AU - Moço, Natália Prearo

AU - de Lima, Moisés Diôgo

AU - Polettini, Jossimara

AU - Miot, Hélio Amante

AU - Corrêa, Camila Renata

AU - Menon, Ramkumar

AU - da Silva, Márcia Guimarães

PY - 2017/11/13

Y1 - 2017/11/13

N2 - Background: Infection induced-inflammation and other risk factors for spontaneous preterm birth (PTB) and preterm premature rupture of membranes (pPROM) may cause a redox imbalance, increasing the release of free radicals and consuming antioxidant defenses. Oxidative stress, in turn, can initiate intracellular signaling cascades that increase the production of pro-inflammatory mediators. The objective of this study was to evaluate the oxidative damage to proteins and antioxidant capacity profiles in amniochorion membranes from preterm birth (PTB) and preterm premature rupture of membranes (pPROM) and to determine the role of histologic chorioamnionitis in this scenario. Methods: We included 27 pregnant women with PTB, 27 pPROM and 30 at term. Protein oxidative damage was assayed by 3-nitrotyrosine (3-NT) and carbonyl levels, using enzyme-linked immunosorbent assay (ELISA) and modified dinitrophenylhydrazine assay (DNPH), respectively. Total antioxidant capacity (TAC) was measured by ELISA. Results: Protein oxidative damage determined by carbonyl levels was lower in PTB group than pPROM and term groups (p < 0.001). PTB group presented higher TAC compared with pPROM and term groups (p = 0.002). Histologic chorioamnionitis did not change either protein oxidative damage or TAC regardless of gestational outcome. Conclusion: These results corroborates previous reports that pPROM and term birth exhibit similarities in oxidative stress- induced senescence and histologic chorioamnionitis does not modulate oxidative stress or antioxidant status.

AB - Background: Infection induced-inflammation and other risk factors for spontaneous preterm birth (PTB) and preterm premature rupture of membranes (pPROM) may cause a redox imbalance, increasing the release of free radicals and consuming antioxidant defenses. Oxidative stress, in turn, can initiate intracellular signaling cascades that increase the production of pro-inflammatory mediators. The objective of this study was to evaluate the oxidative damage to proteins and antioxidant capacity profiles in amniochorion membranes from preterm birth (PTB) and preterm premature rupture of membranes (pPROM) and to determine the role of histologic chorioamnionitis in this scenario. Methods: We included 27 pregnant women with PTB, 27 pPROM and 30 at term. Protein oxidative damage was assayed by 3-nitrotyrosine (3-NT) and carbonyl levels, using enzyme-linked immunosorbent assay (ELISA) and modified dinitrophenylhydrazine assay (DNPH), respectively. Total antioxidant capacity (TAC) was measured by ELISA. Results: Protein oxidative damage determined by carbonyl levels was lower in PTB group than pPROM and term groups (p < 0.001). PTB group presented higher TAC compared with pPROM and term groups (p = 0.002). Histologic chorioamnionitis did not change either protein oxidative damage or TAC regardless of gestational outcome. Conclusion: These results corroborates previous reports that pPROM and term birth exhibit similarities in oxidative stress- induced senescence and histologic chorioamnionitis does not modulate oxidative stress or antioxidant status.

KW - Antioxidant capacity

KW - Histologic chorioamnionitis

KW - Oxidative stress

KW - Preterm birth

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