Histologic detection of multiple blood meals in Phlebotomus duboscqi (Diptera: Psychodidae).

H. Guzman, L. L. Walters, R. B. Tesh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A histologic technique was used to detect multiple hamster blood meals taken by Phlebotomus duboscqi Neveu-Lemaire during a 5-d period. Forty-eight flies were fed two or three blood meals separated by 48, 72, or 120 h and sampled immediately; multiple meals were detected in 27 flies (56%). Double meals separated by 72 h within a single gonotrophic cycle were documented in 11/19 (58%) flies; double meals separated by 120 h were detected in only 4/17 (24%) flies. Triple blood meals taken at 0, 72, and 120 h were detected in 5/12 (42%) flies; all of these flies contained the second and third meals. Early blood meals were detected clearly within later blood meals as a delimited body of dark digested blood, heme (sometimes also with pink undigested blood), the presence of an associated pale pink-staining peritrophic plug, the presence and appearance of the peritrophic membrane surrounding the meals, and a physical space between meals; the first two characteristics were the most important. Development of the ovarian follicles including apparent dilatations was also observable using this histologic technique. The results of this study indicate that the rate of multiple feeding can be determined using histology. The technique would be useful in evaluating the blood feeding frequency of field-caught sand flies in endemic areas of leishmaniasis, bartonellosis, and phleboviruses.

Original languageEnglish (US)
Pages (from-to)890-897
Number of pages8
JournalJournal of Medical Entomology
Volume31
Issue number6
StatePublished - Nov 1994
Externally publishedYes

Fingerprint

Phlebotomus duboscqi
Phlebotomus
Psychodidae
blood meal
Diptera
Meals
blood
Phlebovirus
Histological Techniques
gonotrophic cycle
peritrophic membrane
feeding frequency
Phlebotominae
leishmaniasis
ovarian follicles
heme
hamsters
histology
Bartonella Infections
methodology

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Histologic detection of multiple blood meals in Phlebotomus duboscqi (Diptera : Psychodidae). / Guzman, H.; Walters, L. L.; Tesh, R. B.

In: Journal of Medical Entomology, Vol. 31, No. 6, 11.1994, p. 890-897.

Research output: Contribution to journalArticle

Guzman, H. ; Walters, L. L. ; Tesh, R. B. / Histologic detection of multiple blood meals in Phlebotomus duboscqi (Diptera : Psychodidae). In: Journal of Medical Entomology. 1994 ; Vol. 31, No. 6. pp. 890-897.
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