Histologic response of porcine collagen-coated and uncoated polypropylene grafts in a rabbit vagina model

R. Keith Huffaker, Tristi W. Muir, Arundhati Rao, Shannon S. Baumann, Thomas J. Kuehl, Lisa M. Pierce

    Research output: Contribution to journalArticle

    48 Citations (Scopus)

    Abstract

    Objective: The purpose of this study was to determine whether the host tissue response in the rabbit vagina differs for 2 synthetic graft materials that are used in pelvic reconstructive surgery. Study Design: One strip of porcine collagen-coated or uncoated polypropylene mesh was implanted adjacent to a sham operative site into the posterior vagina of 10 New Zealand white rabbits and harvested 12 weeks later. Rabbits were assigned randomly to group 1 (coated; n = 5), group 2 (uncoated; n = 5), or group 3 (unoperated; n = 4). Full-thickness sagittal sections of posterior vaginal wall and rectum were scored for inflammation, neovascularization, and fibroblastic proliferation. Results: Erosion of grafts did not occur in any animal. Coated and uncoated meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts. Few apoptotic and proliferating cells were seen for both graft types. Conclusion: Both coated and uncoated polypropylene meshes elicit a mild foreign body reaction and minimal fibrotic response without evidence of vaginal epithelial erosion.

    Original languageEnglish (US)
    JournalAmerican Journal of Obstetrics and Gynecology
    Volume198
    Issue number5
    DOIs
    StatePublished - May 2008

    Fingerprint

    Polypropylenes
    Vagina
    Swine
    Collagen
    Rabbits
    Transplants
    Reconstructive Surgical Procedures
    Foreign-Body Reaction
    Rectum
    Fibrosis
    Inflammation

    Keywords

    • Histologic finding
    • pelvic organ prolapse
    • polypropylene mesh
    • rabbit model
    • synthetic graft
    • vagina

    ASJC Scopus subject areas

    • Medicine(all)
    • Obstetrics and Gynecology

    Cite this

    Histologic response of porcine collagen-coated and uncoated polypropylene grafts in a rabbit vagina model. / Huffaker, R. Keith; Muir, Tristi W.; Rao, Arundhati; Baumann, Shannon S.; Kuehl, Thomas J.; Pierce, Lisa M.

    In: American Journal of Obstetrics and Gynecology, Vol. 198, No. 5, 05.2008.

    Research output: Contribution to journalArticle

    Huffaker, R. Keith ; Muir, Tristi W. ; Rao, Arundhati ; Baumann, Shannon S. ; Kuehl, Thomas J. ; Pierce, Lisa M. / Histologic response of porcine collagen-coated and uncoated polypropylene grafts in a rabbit vagina model. In: American Journal of Obstetrics and Gynecology. 2008 ; Vol. 198, No. 5.
    @article{a0bf935434794081be4163df06183977,
    title = "Histologic response of porcine collagen-coated and uncoated polypropylene grafts in a rabbit vagina model",
    abstract = "Objective: The purpose of this study was to determine whether the host tissue response in the rabbit vagina differs for 2 synthetic graft materials that are used in pelvic reconstructive surgery. Study Design: One strip of porcine collagen-coated or uncoated polypropylene mesh was implanted adjacent to a sham operative site into the posterior vagina of 10 New Zealand white rabbits and harvested 12 weeks later. Rabbits were assigned randomly to group 1 (coated; n = 5), group 2 (uncoated; n = 5), or group 3 (unoperated; n = 4). Full-thickness sagittal sections of posterior vaginal wall and rectum were scored for inflammation, neovascularization, and fibroblastic proliferation. Results: Erosion of grafts did not occur in any animal. Coated and uncoated meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts. Few apoptotic and proliferating cells were seen for both graft types. Conclusion: Both coated and uncoated polypropylene meshes elicit a mild foreign body reaction and minimal fibrotic response without evidence of vaginal epithelial erosion.",
    keywords = "Histologic finding, pelvic organ prolapse, polypropylene mesh, rabbit model, synthetic graft, vagina",
    author = "Huffaker, {R. Keith} and Muir, {Tristi W.} and Arundhati Rao and Baumann, {Shannon S.} and Kuehl, {Thomas J.} and Pierce, {Lisa M.}",
    year = "2008",
    month = "5",
    doi = "10.1016/j.ajog.2007.12.029",
    language = "English (US)",
    volume = "198",
    journal = "American Journal of Obstetrics and Gynecology",
    issn = "0002-9378",
    publisher = "Mosby Inc.",
    number = "5",

    }

    TY - JOUR

    T1 - Histologic response of porcine collagen-coated and uncoated polypropylene grafts in a rabbit vagina model

    AU - Huffaker, R. Keith

    AU - Muir, Tristi W.

    AU - Rao, Arundhati

    AU - Baumann, Shannon S.

    AU - Kuehl, Thomas J.

    AU - Pierce, Lisa M.

    PY - 2008/5

    Y1 - 2008/5

    N2 - Objective: The purpose of this study was to determine whether the host tissue response in the rabbit vagina differs for 2 synthetic graft materials that are used in pelvic reconstructive surgery. Study Design: One strip of porcine collagen-coated or uncoated polypropylene mesh was implanted adjacent to a sham operative site into the posterior vagina of 10 New Zealand white rabbits and harvested 12 weeks later. Rabbits were assigned randomly to group 1 (coated; n = 5), group 2 (uncoated; n = 5), or group 3 (unoperated; n = 4). Full-thickness sagittal sections of posterior vaginal wall and rectum were scored for inflammation, neovascularization, and fibroblastic proliferation. Results: Erosion of grafts did not occur in any animal. Coated and uncoated meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts. Few apoptotic and proliferating cells were seen for both graft types. Conclusion: Both coated and uncoated polypropylene meshes elicit a mild foreign body reaction and minimal fibrotic response without evidence of vaginal epithelial erosion.

    AB - Objective: The purpose of this study was to determine whether the host tissue response in the rabbit vagina differs for 2 synthetic graft materials that are used in pelvic reconstructive surgery. Study Design: One strip of porcine collagen-coated or uncoated polypropylene mesh was implanted adjacent to a sham operative site into the posterior vagina of 10 New Zealand white rabbits and harvested 12 weeks later. Rabbits were assigned randomly to group 1 (coated; n = 5), group 2 (uncoated; n = 5), or group 3 (unoperated; n = 4). Full-thickness sagittal sections of posterior vaginal wall and rectum were scored for inflammation, neovascularization, and fibroblastic proliferation. Results: Erosion of grafts did not occur in any animal. Coated and uncoated meshes induced a mild inflammatory response with minimal fibrosis and good host tissue incorporation within the grafts. Few apoptotic and proliferating cells were seen for both graft types. Conclusion: Both coated and uncoated polypropylene meshes elicit a mild foreign body reaction and minimal fibrotic response without evidence of vaginal epithelial erosion.

    KW - Histologic finding

    KW - pelvic organ prolapse

    KW - polypropylene mesh

    KW - rabbit model

    KW - synthetic graft

    KW - vagina

    UR - http://www.scopus.com/inward/record.url?scp=42749097187&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=42749097187&partnerID=8YFLogxK

    U2 - 10.1016/j.ajog.2007.12.029

    DO - 10.1016/j.ajog.2007.12.029

    M3 - Article

    VL - 198

    JO - American Journal of Obstetrics and Gynecology

    JF - American Journal of Obstetrics and Gynecology

    SN - 0002-9378

    IS - 5

    ER -