Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

Natalia S. Carvalho, Antonio F. Moron, Ramkumar Menon, Sergio Cavalheiro, Mauricio M. Barbosa, Herbene J. Milani, Marcia M. Ishigai

    Research output: Contribution to journalArticlepeer-review

    12 Scopus citations

    Abstract

    An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius‑polarization method comparing the suture site with non‑suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t‑test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non‑surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mecha­nism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.

    Original languageEnglish (US)
    Pages (from-to)3732-3736
    Number of pages5
    JournalExperimental and Therapeutic Medicine
    Volume14
    Issue number4
    DOIs
    StatePublished - Oct 2017

    Keywords

    • Fetal membrane healing
    • Fetal membrane repair
    • Myelomeningocele
    • Open fetal surgery
    • Spina bifida

    ASJC Scopus subject areas

    • Immunology and Microbiology (miscellaneous)
    • Cancer Research

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