Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele

Natalia S. Carvalho, Antonio F. Moron, Ramkumar Menon, Sergio Cavalheiro, Mauricio M. Barbosa, Herbene J. Milani, Marcia M. Ishigai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An increased understanding of the reparative process in fetal membrane following surgical techniques may be helpful to decrease the risks to mother and fetus and avoid adverse pregnancy outcomes. The present study discusses histological evaluation of the fetal membrane following open fetal surgery. Chorioamniotic membranes (n=10) were obtained following birth from pregnancies that underwent open fetal surgery for myelomeningocele. The collagen distribution was quantified using picrosirius‑polarization method comparing the suture site with non‑suture site. The differences between the collagen fiber percentages at the two sites was evaluated by the paired t‑test with P<0.05. The mean gestational age of fetal surgery was 26.09±0.3 and 33.81±0.82 weeks at birth. The picrosirius red sign was more intense at the suture site, primarily associated with collagen type 1. Collagen observed in the surgical area was significantly increased (13.22±2.84%) compared with the non‑surgical area (6.16±1.09%; P<0.0001). It was observed that the reparative activity at the suture site of the fetal membrane was characterized by a significant increase in collagen fibers. The findings suggest nascent collagen synthesis, tissue remodeling and repair of suture site, a mecha­nism likely to prevent the amniotic fluid leakage and maintain pregnancy following open fetal surgery.

Original languageEnglish (US)
Pages (from-to)3732-3736
Number of pages5
JournalExperimental and Therapeutic Medicine
Volume14
Issue number4
DOIs
StatePublished - Oct 1 2017

Fingerprint

Meningomyelocele
Collagen
Extraembryonic Membranes
Sutures
Membranes
Parturition
Pregnancy
Amniotic Fluid
Pregnancy Outcome
Collagen Type I
Gestational Age
Fetus

Keywords

  • Fetal membrane healing
  • Fetal membrane repair
  • Myelomeningocele
  • Open fetal surgery
  • Spina bifida

ASJC Scopus subject areas

  • Immunology and Microbiology (miscellaneous)
  • Cancer Research

Cite this

Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. / Carvalho, Natalia S.; Moron, Antonio F.; Menon, Ramkumar; Cavalheiro, Sergio; Barbosa, Mauricio M.; Milani, Herbene J.; Ishigai, Marcia M.

In: Experimental and Therapeutic Medicine, Vol. 14, No. 4, 01.10.2017, p. 3732-3736.

Research output: Contribution to journalArticle

Carvalho, Natalia S. ; Moron, Antonio F. ; Menon, Ramkumar ; Cavalheiro, Sergio ; Barbosa, Mauricio M. ; Milani, Herbene J. ; Ishigai, Marcia M. / Histological evidence of reparative activity in chorioamniotic membrane following open fetal surgery for myelomeningocele. In: Experimental and Therapeutic Medicine. 2017 ; Vol. 14, No. 4. pp. 3732-3736.
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