Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996

Tetsuro Ikegami; Mary Elizabeth G. Miranda; Alan B. Calaor; Daria L. Manalo; Noel J. Miranda; Masahiro Niikura; Masayuki Saijo; Yumi Une; Yasuo Nomura; Ichiro Kurane; Thomas G. Ksiazek; Yasuhiro Yoshikawa; Shigeru Morikawa

doi:10.1538/expanim.51.447

Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996

Tetsuro Ikegami, Mary Elizabeth G. Miranda, Alan B. Calaor, Daria L. Manalo, Noel J. Miranda, Masahiro Niikura, Masayuki Saijo, Yumi Une, Yasuo Nomura, Ichiro Kurane, Thomas G. Ksiazek, Yasuhiro Yoshikawa, Shigeru Morikawa

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18 Scopus citations

Abstract

We investigated the livers, spleens, kidneys and lungs collected from 24 cynomolgus macaques (Macaca fascicularis) naturally infected with Ebola virus subtype Reston (EBO-R) during the Philippine outbreak in 1996, in order to reveal the histopathologic findings. These macaques showed necrotic hepatocytes with inclusions, slight to massive fibrin deposition in splenic cords, depletion of lymphoid cells in the white pulp of the spleen, and fibrin thrombi in some organs. Immunohistochemical analysis using anti-leukocyte antigen L1 antibody revealed an increase in blood-derived macrophages/ monocytes in the livers, kidneys and lungs of EBO-R infected macaques. EBO-R NP antigens were detected in the macrophages/monocytes, endothelial cells and fibroblasts in the liver, spleen, kidney and lung. These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/ monocytes in accordance with the EBO-R propagation in macrophages/monocytes.

Original language	English (US)
Pages (from-to)	447-455
Number of pages	9
Journal	Experimental Animals
Volume	51
Issue number	5
DOIs	https://doi.org/10.1538/expanim.51.447
State	Published - Oct 2002
Externally published	Yes

Keywords

Cynomolgus macaque
Ebola virus
Histopathology
Subtype Reston

ASJC Scopus subject areas

Animal Science and Zoology
General Biochemistry, Genetics and Molecular Biology
General Veterinary

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10.1538/expanim.51.447

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Ikegami, T., Miranda, M. E. G., Calaor, A. B., Manalo, D. L., Miranda, N. J., Niikura, M., Saijo, M., Une, Y., Nomura, Y., Kurane, I., Ksiazek, T. G., Yoshikawa, Y., & Morikawa, S. (2002). Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996. Experimental Animals, 51(5), 447-455. https://doi.org/10.1538/expanim.51.447

Ikegami, T, Miranda, MEG, Calaor, AB, Manalo, DL, Miranda, NJ, Niikura, M, Saijo, M, Une, Y, Nomura, Y, Kurane, I, Ksiazek, TG, Yoshikawa, Y & Morikawa, S 2002, 'Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996', Experimental Animals, vol. 51, no. 5, pp. 447-455. https://doi.org/10.1538/expanim.51.447

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title = "Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996",

abstract = "We investigated the livers, spleens, kidneys and lungs collected from 24 cynomolgus macaques (Macaca fascicularis) naturally infected with Ebola virus subtype Reston (EBO-R) during the Philippine outbreak in 1996, in order to reveal the histopathologic findings. These macaques showed necrotic hepatocytes with inclusions, slight to massive fibrin deposition in splenic cords, depletion of lymphoid cells in the white pulp of the spleen, and fibrin thrombi in some organs. Immunohistochemical analysis using anti-leukocyte antigen L1 antibody revealed an increase in blood-derived macrophages/ monocytes in the livers, kidneys and lungs of EBO-R infected macaques. EBO-R NP antigens were detected in the macrophages/monocytes, endothelial cells and fibroblasts in the liver, spleen, kidney and lung. These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/ monocytes in accordance with the EBO-R propagation in macrophages/monocytes.",

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author = "Tetsuro Ikegami and Miranda, {Mary Elizabeth G.} and Calaor, {Alan B.} and Manalo, {Daria L.} and Miranda, {Noel J.} and Masahiro Niikura and Masayuki Saijo and Yumi Une and Yasuo Nomura and Ichiro Kurane and Ksiazek, {Thomas G.} and Yasuhiro Yoshikawa and Shigeru Morikawa",

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doi = "10.1538/expanim.51.447",

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AU - Ikegami, Tetsuro

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AU - Manalo, Daria L.

AU - Miranda, Noel J.

AU - Niikura, Masahiro

AU - Saijo, Masayuki

AU - Une, Yumi

AU - Nomura, Yasuo

AU - Kurane, Ichiro

AU - Ksiazek, Thomas G.

AU - Yoshikawa, Yasuhiro

AU - Morikawa, Shigeru

PY - 2002/10

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N2 - We investigated the livers, spleens, kidneys and lungs collected from 24 cynomolgus macaques (Macaca fascicularis) naturally infected with Ebola virus subtype Reston (EBO-R) during the Philippine outbreak in 1996, in order to reveal the histopathologic findings. These macaques showed necrotic hepatocytes with inclusions, slight to massive fibrin deposition in splenic cords, depletion of lymphoid cells in the white pulp of the spleen, and fibrin thrombi in some organs. Immunohistochemical analysis using anti-leukocyte antigen L1 antibody revealed an increase in blood-derived macrophages/ monocytes in the livers, kidneys and lungs of EBO-R infected macaques. EBO-R NP antigens were detected in the macrophages/monocytes, endothelial cells and fibroblasts in the liver, spleen, kidney and lung. These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/ monocytes in accordance with the EBO-R propagation in macrophages/monocytes.

AB - We investigated the livers, spleens, kidneys and lungs collected from 24 cynomolgus macaques (Macaca fascicularis) naturally infected with Ebola virus subtype Reston (EBO-R) during the Philippine outbreak in 1996, in order to reveal the histopathologic findings. These macaques showed necrotic hepatocytes with inclusions, slight to massive fibrin deposition in splenic cords, depletion of lymphoid cells in the white pulp of the spleen, and fibrin thrombi in some organs. Immunohistochemical analysis using anti-leukocyte antigen L1 antibody revealed an increase in blood-derived macrophages/ monocytes in the livers, kidneys and lungs of EBO-R infected macaques. EBO-R NP antigens were detected in the macrophages/monocytes, endothelial cells and fibroblasts in the liver, spleen, kidney and lung. These results indicate that EBO-R infection is characterized by systemic coagulopathy and an increase in blood-derived macrophages/ monocytes in accordance with the EBO-R propagation in macrophages/monocytes.

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KW - Subtype Reston

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Histopathology of natural Ebola virus subtype Reston infection in cynomolgus macaques during the Philippine outbreak in 1996

Abstract

Keywords

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