HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

Zhen Yang, Richard Morrison, Carl Oates, Juan Sarria, Janak Patel, Afsoon Habibi, Rana Seyedjafari, Peter Hu, Alan Lennon, Albert Li, Jianli Dong

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7 Scopus citations

Abstract

Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.

Original languageEnglish (US)
Pages (from-to)671-673
Number of pages3
JournalLaboratory Medicine
Volume39
Issue number11
DOIs
StatePublished - 2008

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ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Yang, Z., Morrison, R., Oates, C., Sarria, J., Patel, J., Habibi, A., Seyedjafari, R., Hu, P., Lennon, A., Li, A., & Dong, J. (2008). HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System. Laboratory Medicine, 39(11), 671-673. https://doi.org/10.1309/LMVOVU1XRB9O3JZV