HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System

Zhen Yang, Richard Morrison, Carl Oates, Juan Sarria, Janak Patel, Afsoon Habibi, Rana Seyedjafari, Peter Hu, Alan Lennon, Albert Li, Jianli Dong

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: The Abbott ViroSeq HIV-1 Genotyping System requires a minimum viral load of 2,000 copies/mL. The aim of this study was to evaluate the performance on samples of a viral load <2,000 copies/mL. Method: We performed a retrospective analysis of 780 genotypic tests performed at the University of Texas Medical Branch (UTMB) Molecular Diagnostics Laboratory between April 2007 and January 2008. Results: A quarter of the samples (25.5%, 199/780) had HIV viremia <2,000 copies/mL. Of the 199 specimens with plasma HIV RNA <2,000 copies/mL, genotyping was completed in 111 of 118 (94%) samples with quantifiable HIV RNA, but only in 48 of 81 (59%) specimens with a viral load <75 copies/mL. Conclusion: Our data does not support the conclusion of some investigators that HIV-1 genotypic resistance testing is technically unreliable on low viral load specimens.

Original languageEnglish (US)
Pages (from-to)671-673
Number of pages3
JournalLaboratory Medicine
Volume39
Issue number11
DOIs
StatePublished - 2008

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Viral Load
HIV-1
RNA
HIV
Testing
Plasmas
Molecular Pathology
Viremia
Research Personnel

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System. / Yang, Zhen; Morrison, Richard; Oates, Carl; Sarria, Juan; Patel, Janak; Habibi, Afsoon; Seyedjafari, Rana; Hu, Peter; Lennon, Alan; Li, Albert; Dong, Jianli.

In: Laboratory Medicine, Vol. 39, No. 11, 2008, p. 671-673.

Research output: Contribution to journalArticle

Yang, Z, Morrison, R, Oates, C, Sarria, J, Patel, J, Habibi, A, Seyedjafari, R, Hu, P, Lennon, A, Li, A & Dong, J 2008, 'HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System', Laboratory Medicine, vol. 39, no. 11, pp. 671-673. https://doi.org/10.1309/LMVOVU1XRB9O3JZV
Yang, Zhen ; Morrison, Richard ; Oates, Carl ; Sarria, Juan ; Patel, Janak ; Habibi, Afsoon ; Seyedjafari, Rana ; Hu, Peter ; Lennon, Alan ; Li, Albert ; Dong, Jianli. / HIV-1 genotypic resistance testing on low viral load specimens using the Abbott ViroSeq HIV-1 Genotyping System. In: Laboratory Medicine. 2008 ; Vol. 39, No. 11. pp. 671-673.
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AU - Patel, Janak

AU - Habibi, Afsoon

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AU - Hu, Peter

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