HIV envelope suppresses CD4+ T cell activation independent of T regulatory cells

Haitao Hu, Kathy Fernando, Houping Ni, Drew Weissman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We previously demonstrated that HIV envelope glycoprotein (Env), delivered in the form of a vaccine and expressed by dendritic cells or 293T cells, could suppress Ag-stimulated CD4+ T cell proliferation. The mechanism remains to be identified but is dependent on CD4 and independent of coreceptor binding. Recently, CD4+ regulatory T (Treg) cells were found to inhibit protective anti-HIV CD4+ and CD8+ T cell responses. However, the role of Tregs in HIV remains highly controversial. HIV Env is a potent immune inhibitory molecule that interacts with host CD4 + cells, including Treg cells. Using an in vitro model, we investigated whether Treg cells are involved in Env-induced suppression of CD4+ T cell proliferation, and whether Env directly affects the functional activity of Treg cells. Our data shows that exposure of human CD4+ T cells to Env neither induced a higher frequency nor a more activated phenotype of Treg cells. Depletion of CD25+ Treg cells from PBMC did not overcome the Env-induced suppression of CD4+ T cell proliferation, demonstrating that CD25+FoxP3+ Treg cells are not involved in Envinduced suppression of CD4+ T cell proliferation. In addition, we extend our observation that similar to Env expressed on cells, Env present on virions also suppresses CD4+ T cell proliferation.

Original languageEnglish (US)
Pages (from-to)5593-5600
Number of pages8
JournalJournal of Immunology
Volume180
Issue number8
StatePublished - Apr 15 2008
Externally publishedYes

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Regulatory T-Lymphocytes
HIV
T-Lymphocytes
Cell Proliferation
env Gene Products
HEK293 Cells
Virion
Dendritic Cells
Vaccines
Phenotype

ASJC Scopus subject areas

  • Immunology

Cite this

HIV envelope suppresses CD4+ T cell activation independent of T regulatory cells. / Hu, Haitao; Fernando, Kathy; Ni, Houping; Weissman, Drew.

In: Journal of Immunology, Vol. 180, No. 8, 15.04.2008, p. 5593-5600.

Research output: Contribution to journalArticle

Hu, H, Fernando, K, Ni, H & Weissman, D 2008, 'HIV envelope suppresses CD4+ T cell activation independent of T regulatory cells', Journal of Immunology, vol. 180, no. 8, pp. 5593-5600.
Hu, Haitao ; Fernando, Kathy ; Ni, Houping ; Weissman, Drew. / HIV envelope suppresses CD4+ T cell activation independent of T regulatory cells. In: Journal of Immunology. 2008 ; Vol. 180, No. 8. pp. 5593-5600.
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