HIV induces homing of resting T lymphocytes to lymph nodes

Liqiang Wang, Christopher W. Robb, Miles W. Cloyd

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98% of the lymphocytes in vivo at any given time are resting and are not permissive for productive infection. We found that exposure of resting CD4+ T lymphocytes to HIV-1 transiently upregulated expression of cell surface CD62L (L-selectin), the receptor for homing to lymph nodes, with concomitant enhanced ability of these cells to bind to lymph node high endothelial venules in an ex vivo homing assay (increased ~ 12-fold, P < 0.001) and to home from the blood into lymph nodes following intravenous injection into SCID mice. This suggested the possibility that decreases in numbers of CD4+ T Iymphocytes in the blood of HIV-1-infected subjects may reflect enhanced homing of abortively infected, resting lymphocytes into lymph nodes rather than direct virus replication in and killing of these cells, and may explain development of lymphadenopathy at a time when numbers of CD4+ T lymphocytes in the blood fall.

Original languageEnglish (US)
Pages (from-to)141-152
Number of pages12
JournalVirology
Volume228
Issue number2
DOIs
StatePublished - Feb 17 1997

Fingerprint

Lymph Nodes
HIV
T-Lymphocytes
HIV-1
Lymphocytes
Lymphocyte Homing Receptors
L-Selectin
SCID Mice
Venules
Virus Replication
Infection
Intravenous Injections

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

HIV induces homing of resting T lymphocytes to lymph nodes. / Wang, Liqiang; Robb, Christopher W.; Cloyd, Miles W.

In: Virology, Vol. 228, No. 2, 17.02.1997, p. 141-152.

Research output: Contribution to journalArticle

Wang, L, Robb, CW & Cloyd, MW 1997, 'HIV induces homing of resting T lymphocytes to lymph nodes', Virology, vol. 228, no. 2, pp. 141-152. https://doi.org/10.1006/viro.1996.8397
Wang, Liqiang ; Robb, Christopher W. ; Cloyd, Miles W. / HIV induces homing of resting T lymphocytes to lymph nodes. In: Virology. 1997 ; Vol. 228, No. 2. pp. 141-152.
@article{ba7f1af5753e438ea45a73bd8b49ace9,
title = "HIV induces homing of resting T lymphocytes to lymph nodes",
abstract = "In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98{\%} of the lymphocytes in vivo at any given time are resting and are not permissive for productive infection. We found that exposure of resting CD4+ T lymphocytes to HIV-1 transiently upregulated expression of cell surface CD62L (L-selectin), the receptor for homing to lymph nodes, with concomitant enhanced ability of these cells to bind to lymph node high endothelial venules in an ex vivo homing assay (increased ~ 12-fold, P < 0.001) and to home from the blood into lymph nodes following intravenous injection into SCID mice. This suggested the possibility that decreases in numbers of CD4+ T Iymphocytes in the blood of HIV-1-infected subjects may reflect enhanced homing of abortively infected, resting lymphocytes into lymph nodes rather than direct virus replication in and killing of these cells, and may explain development of lymphadenopathy at a time when numbers of CD4+ T lymphocytes in the blood fall.",
author = "Liqiang Wang and Robb, {Christopher W.} and Cloyd, {Miles W.}",
year = "1997",
month = "2",
day = "17",
doi = "10.1006/viro.1996.8397",
language = "English (US)",
volume = "228",
pages = "141--152",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - HIV induces homing of resting T lymphocytes to lymph nodes

AU - Wang, Liqiang

AU - Robb, Christopher W.

AU - Cloyd, Miles W.

PY - 1997/2/17

Y1 - 1997/2/17

N2 - In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98% of the lymphocytes in vivo at any given time are resting and are not permissive for productive infection. We found that exposure of resting CD4+ T lymphocytes to HIV-1 transiently upregulated expression of cell surface CD62L (L-selectin), the receptor for homing to lymph nodes, with concomitant enhanced ability of these cells to bind to lymph node high endothelial venules in an ex vivo homing assay (increased ~ 12-fold, P < 0.001) and to home from the blood into lymph nodes following intravenous injection into SCID mice. This suggested the possibility that decreases in numbers of CD4+ T Iymphocytes in the blood of HIV-1-infected subjects may reflect enhanced homing of abortively infected, resting lymphocytes into lymph nodes rather than direct virus replication in and killing of these cells, and may explain development of lymphadenopathy at a time when numbers of CD4+ T lymphocytes in the blood fall.

AB - In vivo infection with human immunodeficiency virus type 1 (HIV-1) leads to gradual depletion of CD4+ T lymphocytes from the peripheral blood and later from the lymphoid organs. The mechanism of CD4 cell depletion is not known. HIV can only replicate in dividing lymphocytes, but greater than 98% of the lymphocytes in vivo at any given time are resting and are not permissive for productive infection. We found that exposure of resting CD4+ T lymphocytes to HIV-1 transiently upregulated expression of cell surface CD62L (L-selectin), the receptor for homing to lymph nodes, with concomitant enhanced ability of these cells to bind to lymph node high endothelial venules in an ex vivo homing assay (increased ~ 12-fold, P < 0.001) and to home from the blood into lymph nodes following intravenous injection into SCID mice. This suggested the possibility that decreases in numbers of CD4+ T Iymphocytes in the blood of HIV-1-infected subjects may reflect enhanced homing of abortively infected, resting lymphocytes into lymph nodes rather than direct virus replication in and killing of these cells, and may explain development of lymphadenopathy at a time when numbers of CD4+ T lymphocytes in the blood fall.

UR - http://www.scopus.com/inward/record.url?scp=0031575537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031575537&partnerID=8YFLogxK

U2 - 10.1006/viro.1996.8397

DO - 10.1006/viro.1996.8397

M3 - Article

VL - 228

SP - 141

EP - 152

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -