TY - JOUR
T1 - HIV Isolation from Plasma of HIV-Infected Persons
AU - Falk, Lawrence A.
AU - Paul, Deborah
AU - Landay, Alan
AU - Kessler, Harold
PY - 1987/6/11
Y1 - 1987/6/11
N2 - To the Editor: Isolation of HIV from lymphocytes of HIV-infected persons by cocultivation methods has been routinely successful,1,2 but direct isolation of infectious virus from serum or plasma has met with only limited success.3,4 For our studies, heparinized blood was obtained from 27 HIV-infected persons. Plasma was assayed for HIV antigen (predominantly p24) by a commercially available enzyme immunosorbent assay (Abbott),5 and antibodies to p24 (core) and gp41 (envelope) proteins were assayed by competitive enzyme immunosorbent assays using recombinant p24 and gp41 antigens.6 HIV was isolated from patients' lymphocytes by cocultivation methods and from filtered plasma (0.45 μm); 1.0 ml….
AB - To the Editor: Isolation of HIV from lymphocytes of HIV-infected persons by cocultivation methods has been routinely successful,1,2 but direct isolation of infectious virus from serum or plasma has met with only limited success.3,4 For our studies, heparinized blood was obtained from 27 HIV-infected persons. Plasma was assayed for HIV antigen (predominantly p24) by a commercially available enzyme immunosorbent assay (Abbott),5 and antibodies to p24 (core) and gp41 (envelope) proteins were assayed by competitive enzyme immunosorbent assays using recombinant p24 and gp41 antigens.6 HIV was isolated from patients' lymphocytes by cocultivation methods and from filtered plasma (0.45 μm); 1.0 ml….
UR - http://www.scopus.com/inward/record.url?scp=0023197667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023197667&partnerID=8YFLogxK
U2 - 10.1056/NEJM198706113162413
DO - 10.1056/NEJM198706113162413
M3 - Letter
C2 - 3473279
AN - SCOPUS:0023197667
SN - 0028-4793
VL - 316
SP - 1547
EP - 1548
JO - New England Journal of Medicine
JF - New England Journal of Medicine
IS - 24
ER -