HLA-DQ6 transgenic mice resistance to experimental autoimmune myasthenia gravis is linked to reduced acetylcholine receptor-specific IFN-γ, IL-2 and IL-10 production

Mathilde A. Poussin, Elzbieta Goluszko, Chella S. David, Juan U. Franco, Premkumar Christadoss

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To comprehend the reduced susceptibitity of HLA-DQ6 transgenic mice in comparison with HLA-DQ8 mice, to experimental autoimmune myasthenia gravis (EAMG), we immunized them with acetylcholine receptor (AChR) and examined in vitro, the proliferative and cytokine responses to AChR. When immunized with AChR and examined for AChR-specific lymphocyte responses to AChR, EAMG-resistant DQ6 mice exhibited significantly reduced in vitro lymphoproliferative and cytokine responses to AChR, compared to DQ8 mice. The differences in susceptibility were not linked to a difference in peptide recognition by AChR-specific lymphocytes. AChR T cell epitope mapping showed that both DQ6 and DQ8 responded to the same epitopes, although to varying degrees. Resistance of DQ6 transgenic mice to EAMG was linked to a dramatic suppression of AChR-specific IFN-γ, IL-2 and IL-10 productions by AChR-primed lymph node cells.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalJournal of Autoimmunity
Volume17
Issue number3
DOIs
StatePublished - Nov 1 2001

Fingerprint

Autoimmune Experimental Myasthenia Gravis
Cholinergic Receptors
Interleukin-10
Transgenic Mice
Interleukin-2
HLA-DQ6 antigen
Lymphocytes
Cytokines
Epitope Mapping
T-Lymphocyte Epitopes
Epitopes

Keywords

  • Autoimmunity
  • Cytokines
  • MHC
  • Myasthenia gravis
  • Transgenic

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

HLA-DQ6 transgenic mice resistance to experimental autoimmune myasthenia gravis is linked to reduced acetylcholine receptor-specific IFN-γ, IL-2 and IL-10 production. / Poussin, Mathilde A.; Goluszko, Elzbieta; David, Chella S.; Franco, Juan U.; Christadoss, Premkumar.

In: Journal of Autoimmunity, Vol. 17, No. 3, 01.11.2001, p. 175-180.

Research output: Contribution to journalArticle

Poussin, Mathilde A. ; Goluszko, Elzbieta ; David, Chella S. ; Franco, Juan U. ; Christadoss, Premkumar. / HLA-DQ6 transgenic mice resistance to experimental autoimmune myasthenia gravis is linked to reduced acetylcholine receptor-specific IFN-γ, IL-2 and IL-10 production. In: Journal of Autoimmunity. 2001 ; Vol. 17, No. 3. pp. 175-180.
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