Holliday junction cleavage by yeast Rad1 protein

Yvette Habraken, Patrick Sung, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In Saccharomyces cerevisiae, of the many genes required for excision repair of ultraviolet-damaged DNA, only RAD1 and RAD10 also function in genetic recombination. Complex formation between the RAD1 and RAD10 gene products activates an endonucleolytic function that nicks single-stranded DNA and negatively supercoiled double-stranded DNA. To characterize the recombination role of the proteins Rad1 and Rad10, we have investigated their interaction with the Holliday junction, a four-stranded structure that results from single-stranded crossover between two duplex DNA molecules and whose resolution is obligatory for the generation of mature recombinants. We show that Rad1 binds specifically to a Holliday junction and, in the presence of magnesium, catalyses the endonucleolytic cleavage of the junction. Junction cleavage by Rad1 proceeds sufficiently without Rad10, thus identifying Rad1 as the catalytic subunit of Rad1/Rad10 endonuclease.

Original languageEnglish (US)
Pages (from-to)531-534
Number of pages4
JournalNature
Volume371
Issue number6497
DOIs
StatePublished - Oct 6 1994

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Cruciform DNA
Fungal Proteins
Genetic Recombination
DNA
Endonucleases
Single-Stranded DNA
DNA Repair
Magnesium
Genes
Saccharomyces cerevisiae
Catalytic Domain
Proteins

ASJC Scopus subject areas

  • General

Cite this

Holliday junction cleavage by yeast Rad1 protein. / Habraken, Yvette; Sung, Patrick; Prakash, Louise; Prakash, Satya.

In: Nature, Vol. 371, No. 6497, 06.10.1994, p. 531-534.

Research output: Contribution to journalArticle

Habraken, Yvette ; Sung, Patrick ; Prakash, Louise ; Prakash, Satya. / Holliday junction cleavage by yeast Rad1 protein. In: Nature. 1994 ; Vol. 371, No. 6497. pp. 531-534.
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