Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3

Kizhake V. Soman, Terumi Midoro-Horiuti, Josephine C. Ferreon, Randall M. Goldblum, Edward G. Brooks, Alexander Kurosky, Werner Braun, Catherine H. Schein

Research output: Contribution to journalArticle

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Abstract

The Jun a 3 protein from mountain cedar (Juniperus ashei) pollen, a member of group 5 of the family of plant pathogenesis-related proteins (PR-proteins), reacts with serum IgE from patients with cedar hypersensitivity. We used the crystal structures of two other proteins of this group, thaumatin and an antifungal protein from tobacco, both ~50% identical in sequence to Jun a 3, as templates to build homology models for the allergen. The in-house programs EXDIS and FANTOM were used to extract distance and dihedral angle constraints from the Protein Data Bank files and determine energy-minimized structures. The mean backbone deviations for the energy-refined model structures from either of the templates is <1 Å, their conformational energies are low, and their stereochemical properties (determined with PROCHECK) are acceptable. The circular dichroism spectrum of Jun a 3 is consistent with the postulated β-sheet core. Tryptic fragments of Jun a 3 that reacted with IgE from allergic patients all mapped to one helical/loop surface of the models. The Jun a 3 models have features common to aerosol allergens from completely different protein families, suggesting that tertiary structural elements may mediate the triggering of an allergic response.

Original languageEnglish (US)
Pages (from-to)1601-1609
Number of pages9
JournalBiophysical Journal
Volume79
Issue number3
StatePublished - 2000

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Allergens
Immunoglobulin E
Epitopes
Proteins
Juniperus
Information Storage and Retrieval
Circular Dichroism
Pollen
Aerosols
Tobacco
Hypersensitivity
Databases
Serum

ASJC Scopus subject areas

  • Biophysics

Cite this

Soman, K. V., Midoro-Horiuti, T., Ferreon, J. C., Goldblum, R. M., Brooks, E. G., Kurosky, A., ... Schein, C. H. (2000). Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3. Biophysical Journal, 79(3), 1601-1609.

Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3. / Soman, Kizhake V.; Midoro-Horiuti, Terumi; Ferreon, Josephine C.; Goldblum, Randall M.; Brooks, Edward G.; Kurosky, Alexander; Braun, Werner; Schein, Catherine H.

In: Biophysical Journal, Vol. 79, No. 3, 2000, p. 1601-1609.

Research output: Contribution to journalArticle

Soman, KV, Midoro-Horiuti, T, Ferreon, JC, Goldblum, RM, Brooks, EG, Kurosky, A, Braun, W & Schein, CH 2000, 'Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3', Biophysical Journal, vol. 79, no. 3, pp. 1601-1609.
Soman, Kizhake V. ; Midoro-Horiuti, Terumi ; Ferreon, Josephine C. ; Goldblum, Randall M. ; Brooks, Edward G. ; Kurosky, Alexander ; Braun, Werner ; Schein, Catherine H. / Homology modeling and characterization of IgE binding epitopes of mountain cedar allergen Jun a 3. In: Biophysical Journal. 2000 ; Vol. 79, No. 3. pp. 1601-1609.
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