Hormone-dependent regulation of chicken progesterone receptor deoxyribonucleic acid binding and phosphorylation

Larry Denner, N. L. Weigel, W. T. Schrader, B. W. O'Malley

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

To further understand the structure-function relationships of the chicken oviduct progesterone receptor, the effects of in vivo and in situ progesterone treatment were studied. Immunoprecipitated receptors isolated from oviduct slices incubated in the presence of H3 32PO4 exhibited hormone-dependent phosphorylation. This was correlated with an increase in the apparent mol wt of receptors when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased DNA binding of total cytosolic receptors. Further, in vivo progesterone treatment resulted in dissociation of both the A and B receptor forms from nonhormone-binding proteins (such as heat shock protein-90) in vitro when analyzed by sucrose gradient ultracentrifugation. The 4S and 8S receptors were separated by phosphocellulose column chromatography, treated with ammonium sulfate to convert all receptors to DNA-binding forms, and analyzed for binding to DNA cellulose. The 4S receptor produced as a consequence of in vivo hormone treatment had a 3.35-fold higher affinity for DNA and bound to about a 3-fold greater extent than receptor that did not show altered interaction with other proteins. Thus, in vivo progesterone treatment results in increased receptor phosphorylation, altered interaction with heat shock protein-90, and increased DNA binding.

Original languageEnglish (US)
Pages (from-to)3051-3058
Number of pages8
JournalEndocrinology
Volume125
Issue number6
StatePublished - 1989
Externally publishedYes

Fingerprint

Progesterone Receptors
Progesterone
HSP90 Heat-Shock Proteins
Chickens
Oviducts
Phosphorylation
Hormones
DNA
Ultracentrifugation
Ammonium Sulfate
Sodium Dodecyl Sulfate
Sucrose
Chromatography
Polyacrylamide Gel Electrophoresis
Carrier Proteins
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Hormone-dependent regulation of chicken progesterone receptor deoxyribonucleic acid binding and phosphorylation. / Denner, Larry; Weigel, N. L.; Schrader, W. T.; O'Malley, B. W.

In: Endocrinology, Vol. 125, No. 6, 1989, p. 3051-3058.

Research output: Contribution to journalArticle

Denner, Larry ; Weigel, N. L. ; Schrader, W. T. ; O'Malley, B. W. / Hormone-dependent regulation of chicken progesterone receptor deoxyribonucleic acid binding and phosphorylation. In: Endocrinology. 1989 ; Vol. 125, No. 6. pp. 3051-3058.
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