Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC

Akira Asai, Yusuke Tsuchimoto, Hideko Ohama, Shinya Fukunishi, Yasuhiro Tsuda, Makiko Kobayashi, Kazuhide Higuchi, Fujio Suzuki

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Despite major advances in curative and palliative approaches, hepatocellular carcinoma (HCC) is still the third leading cause of cancer-related death worldwide. M1 macrophages (Mφ) play a key role in host antitumor defenses in HCC. In our study, CD14+ cells were isolated from the peripheral blood of four groups of HCC patients (group-1, patients with stage 0 HCC; group-2, patients with stage A HCC; group-3, patients with stage B HCC; and group-4, patients with stage C HCC) and characterized phenotypically. Then, CD14+ cells from group-2 and group-3 HCC patients were induced to polarize and tested for their antitumor abilities in a chimera model of HCC patients. Human HCCs (HepG2 solid tumors) grew in a chimera model of group-3 patients (group-3 HCC chimeras) but not in a chimera model of group-2 patients (group-2 HCC chimeras). In response to HCC antigens, the majority of CD14+ cells from group-2 patients (group-2 CD14+ cells) switched to the M1 phenotype (IL-12+IL-10iNOS+cells), whereas the majority of CD14+ cells from group-3 patients (group-3 CD14+ cells) did not switch to the M1 phenotype and continued to express M2b phenotypic properties (IL-12IL-10+CCL1+iNOScells). Group-3 CD14+ cells showed M1Mφ polarization after treatment with CCL1 antisense oligodeoxynucleotide (ODN). Therefore, our study indicates that anti-HCC defenses of group-3 HCC chimeras are improved after CCL1 antisense ODN treatment.

Original languageEnglish (US)
Article numbere1299301
JournalOncoImmunology
Volume6
Issue number4
DOIs
StatePublished - Apr 3 2017

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Keywords

  • Antisense oligodexynuclotide
  • CCL1
  • hepatocellular carcinoma
  • host antitumor resistance
  • macrophage

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology

Cite this

Asai, A., Tsuchimoto, Y., Ohama, H., Fukunishi, S., Tsuda, Y., Kobayashi, M., Higuchi, K., & Suzuki, F. (2017). Host antitumor resistance improved by the macrophage polarization in a chimera model of patients with HCC. OncoImmunology, 6(4), [e1299301]. https://doi.org/10.1080/2162402X.2017.1299301