Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts

J. F. Wright, A. Kurosky, E. L G Pryzdial, S. Wasi

Research output: Contribution to journalArticle

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Abstract

A significant amount of host cellular annexin II was found to be associated with human cytomegalovirus isolated from cultured human fibroblasts (~1,160 molecules per virion). This composition was established by four different analytical approaches that included (i) Western blot (Immunoblot) analysis of gradient-purified virions with a monoclonal antibody specific for annexin II, (ii) peptide mapping and sequence analysis of virus- associated proteins and proteins dissociated from virus following EDTA treatment, (iii) electron microscopic immunocytochemistry of gradient- purified virions, and (iv) labeling of virus-associated proteins by lactoperoxidase-catalyzed radioiodination. These results indicated that annexin II was primarily localized to the viral surface, where it bound in a divalent cation-dependent manner. In functional experiments, a rabbit antiserum raised against annexin II inhibited cytomegalovirus plaque formation in human foreskin fibroblast monolayers in a concentration- dependent manner. Cumulatively, these studies demonstrate an association of host annexin II with cytomegalovirus particles and provide evidence for the involvement of this cellular protein in virus infectivity.

Original languageEnglish (US)
Pages (from-to)4784-4791
Number of pages8
JournalJournal of Virology
Volume69
Issue number8
StatePublished - 1995
Externally publishedYes

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Annexin A2
Cytomegalovirus
fibroblasts
Fibroblasts
virion
viruses
Virion
Viruses
proteins
Human herpesvirus 5
peptide mapping
Proteins
Western Blotting
immunocytochemistry
Lactoperoxidase
Foreskin
Peptide Mapping
Protein Sequence Analysis
Divalent Cations
antiserum

ASJC Scopus subject areas

  • Immunology

Cite this

Wright, J. F., Kurosky, A., Pryzdial, E. L. G., & Wasi, S. (1995). Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts. Journal of Virology, 69(8), 4784-4791.

Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts. / Wright, J. F.; Kurosky, A.; Pryzdial, E. L G; Wasi, S.

In: Journal of Virology, Vol. 69, No. 8, 1995, p. 4784-4791.

Research output: Contribution to journalArticle

Wright, JF, Kurosky, A, Pryzdial, ELG & Wasi, S 1995, 'Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts', Journal of Virology, vol. 69, no. 8, pp. 4784-4791.
Wright, J. F. ; Kurosky, A. ; Pryzdial, E. L G ; Wasi, S. / Host cellular annexin II is associated with cytomegalovirus particles isolated from cultured human fibroblasts. In: Journal of Virology. 1995 ; Vol. 69, No. 8. pp. 4784-4791.
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