Host genetic polymorphisms in human immunodeficiency virus-related neurologic disease

Ramon Diaz-Arrastia, Yunhua Gong, Cynthia J. Kelly, Benjamin Gelman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The objective of this study was to determine whether host genetic polymorphisms influence the risk of developing human immunodeficiency virus (HIV) encephalitis and vacuolar myelopathy. Allelic association studies were carried out with common polymorphisms in candidate genes that are postulated to play a role in the pathogenesis of HIV-related neurologic complications. The authors studied brains and spinal cords from 270 patients who died of acquired immunodeficiency syndrome (AIDS) from 1989 to 1996. All had complete gross and microscopic pathologic evaluations, and the presence of microglial nodules, multinucleated giant cells, myelin pallor, and vacuolar myelopathy was assessed by an experienced neuropathologist who was blinded to the genotype. DNA was extracted from frozen brain samples, and determination of the presence of the APOE4, TNF-2, IL-1B*2, ILIRN*2 polymorphisms was determined by polymerase chain reaction (PCR) and restricted fragment length polymorphism (RFLP) mapping. The authors did not detect a consistent association between inheritance of candidate polymorphic alleles and the pathologic findings of HIV encephalitis or vacuolar myelopathy. Allelic association studies with candidate genes are powerful techniques that have the potential to contribute to understanding the pathophysiology of HIV-related neurodegeneration. This preliminary study, although including a substantial number of patients, was not sufficiently powered to exclude a modest but clinically significant effects. Future studies will require much larger sample sizes and technical advances to allow screening at larger number of candidate loci.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalJournal of NeuroVirology
Volume10
Issue numberSUPPL. 1
DOIs
StatePublished - 2004

Fingerprint

Genetic Polymorphisms
Nervous System Diseases
Spinal Cord Diseases
HIV
Encephalitis
Apolipoprotein E4
Pallor
Brain
Genetic Association Studies
Giant Cells
Myelin Sheath
Sample Size
Nervous System
Interleukin-2
Spinal Cord
Acquired Immunodeficiency Syndrome
Alleles
Genotype
Polymerase Chain Reaction
DNA

Keywords

  • Apolipoprotein E
  • Interleukin-1
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

Cite this

Host genetic polymorphisms in human immunodeficiency virus-related neurologic disease. / Diaz-Arrastia, Ramon; Gong, Yunhua; Kelly, Cynthia J.; Gelman, Benjamin.

In: Journal of NeuroVirology, Vol. 10, No. SUPPL. 1, 2004, p. 67-73.

Research output: Contribution to journalArticle

Diaz-Arrastia, Ramon ; Gong, Yunhua ; Kelly, Cynthia J. ; Gelman, Benjamin. / Host genetic polymorphisms in human immunodeficiency virus-related neurologic disease. In: Journal of NeuroVirology. 2004 ; Vol. 10, No. SUPPL. 1. pp. 67-73.
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