Host phospholipase C-γ1 impairs phagocytosis and killing of mycobacteria by J774A.1 murine macrophages

Ruchi Paroha, Rashmi Chourasia, Rupal Rai, Awanish Kumar, Ashish K. Vyas, Shivendra K. Chaurasiya, Anirudh K. Singh

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Macrophages represent the first line of defense against invading Mycobacterium tuberculosis (Mtb). In order to enhance intracellular survival, Mtb targets various components of the host signaling pathways to limit macrophage functions. The outcome of Mtb infection depends on various factors derived from both host and pathogen. A detailed understanding of such factors operating during interaction of the pathogen with the host is a prerequisite for designing new approaches for combating mycobacterial infections. This work analyzed the role of host phospholipase C-γ1 (PLC-γ1) in regulating mycobacterial uptake and killing by J774A.1 murine macrophages. Small interfering RNA mediated knockdown of PLC-γ1 increased internalization and reduced the intracellular survival of both Mtb and Mycobacterium smegmatis (MS) by macrophages. Down-regulation of the host PLC-γ1 was observed during the course of mycobacterial infection within these macrophages. Finally, Mtb infection also suppressed the expression of pro-inflammatory cytokine tumor necrosis factor-α and chemokine (C-C motif) ligand 5 (RANTES) which was restored by knocking down PLC-γ1 in J774A.1 cells. These observations suggest a role of host PLC-γ1 in the uptake and killing of mycobacteria by murine macrophages.

Original languageEnglish (US)
Pages (from-to)694-702
Number of pages9
JournalMICROBIOLOGY and IMMUNOLOGY
Volume64
Issue number10
DOIs
StatePublished - Oct 1 2020
Externally publishedYes

Keywords

  • Mycobacterium tuberculosis
  • cytokines
  • intracellular survival
  • macrophage response
  • phospholipase C-γ1

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Fingerprint

Dive into the research topics of 'Host phospholipase C-γ1 impairs phagocytosis and killing of mycobacteria by J774A.1 murine macrophages'. Together they form a unique fingerprint.

Cite this