HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum

Xiao-Ming Wang, Shu Hong Guan, Rong Xia Liu, Jiang Hao Sun, Yan Liang, Min Yang, Wei Wang, Kai Shun Bi, De An Guo

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.

Original languageEnglish (US)
Pages (from-to)1185-1190
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume43
Issue number3
DOIs
StatePublished - Feb 19 2007
Externally publishedYes

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Keywords

  • Ganoderma lucidum
  • HPLC
  • Rat urine
  • Total triterpenoids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

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