HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum

Xiao-Ming Wang, Shu Hong Guan, Rong Xia Liu, Jiang Hao Sun, Yan Liang, Min Yang, Wei Wang, Kai Shun Bi, De An Guo

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.

Original languageEnglish (US)
Pages (from-to)1185-1190
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume43
Issue number3
DOIs
StatePublished - Feb 19 2007
Externally publishedYes

Fingerprint

Reishi
High performance liquid chromatography
Oral Administration
Rats
High Pressure Liquid Chromatography
Urine
Hydrochloric Acid
Acidification
Methylene Chloride
Hydrocortisone
Assays
Flow rate
Recovery
Wavelength
ganoderic acid C2
ethyl acetate
phosphoric acid
acetonitrile

Keywords

  • Ganoderma lucidum
  • HPLC
  • Rat urine
  • Total triterpenoids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. / Wang, Xiao-Ming; Guan, Shu Hong; Liu, Rong Xia; Sun, Jiang Hao; Liang, Yan; Yang, Min; Wang, Wei; Bi, Kai Shun; Guo, De An.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 43, No. 3, 19.02.2007, p. 1185-1190.

Research output: Contribution to journalArticle

Wang, Xiao-Ming ; Guan, Shu Hong ; Liu, Rong Xia ; Sun, Jiang Hao ; Liang, Yan ; Yang, Min ; Wang, Wei ; Bi, Kai Shun ; Guo, De An. / HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. In: Journal of Pharmaceutical and Biomedical Analysis. 2007 ; Vol. 43, No. 3. pp. 1185-1190.
@article{007d00763e854930a31eb056f8ea110d,
title = "HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum",
abstract = "A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03{\%} aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9{\%} and the extraction recovery of this method was higher than 90{\%}. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.",
keywords = "Ganoderma lucidum, HPLC, Rat urine, Total triterpenoids",
author = "Xiao-Ming Wang and Guan, {Shu Hong} and Liu, {Rong Xia} and Sun, {Jiang Hao} and Yan Liang and Min Yang and Wei Wang and Bi, {Kai Shun} and Guo, {De An}",
year = "2007",
month = "2",
day = "19",
doi = "10.1016/j.jpba.2006.10.010",
language = "English (US)",
volume = "43",
pages = "1185--1190",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum

AU - Wang, Xiao-Ming

AU - Guan, Shu Hong

AU - Liu, Rong Xia

AU - Sun, Jiang Hao

AU - Liang, Yan

AU - Yang, Min

AU - Wang, Wei

AU - Bi, Kai Shun

AU - Guo, De An

PY - 2007/2/19

Y1 - 2007/2/19

N2 - A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.

AB - A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.

KW - Ganoderma lucidum

KW - HPLC

KW - Rat urine

KW - Total triterpenoids

UR - http://www.scopus.com/inward/record.url?scp=33846653132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846653132&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2006.10.010

DO - 10.1016/j.jpba.2006.10.010

M3 - Article

VL - 43

SP - 1185

EP - 1190

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 3

ER -