Abstract
A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90:10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C18 column (250 mm × 4.6 mm, 5 μm) at 35 °C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time.
Original language | English (US) |
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Pages (from-to) | 1185-1190 |
Number of pages | 6 |
Journal | Journal of Pharmaceutical and Biomedical Analysis |
Volume | 43 |
Issue number | 3 |
DOIs | |
State | Published - Feb 19 2007 |
Externally published | Yes |
Keywords
- Ganoderma lucidum
- HPLC
- Rat urine
- Total triterpenoids
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry