HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract

Xiao-Ming Wang, Rongxia Liu, Jianghao Sun, Shuhong Guan, Min Yang, Kaishun Bi, Dean Guo

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Four major triterpenoids (ganoderic acids C2, B, K and H) in rat plasma after oral administration of G. lucidum extract were analyzed quantitatively by high-performance liquid chromatography (HPLC). Plasma samples taken from rats were acidified with hydrochloric acid and extracted with dichloromethane-ethyl acetate (90:10). The chromatographic separation was achieved on an Agilent Zorbax SB-C18 column (250 × 4.6 mm, 5 μm) at 35°C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/ v), at a flow rate of 1.0 mL/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. All calibration curves showed good linearity (r2 > 0.99) within test ranges. The relative deviation of this method was less than 10% for intra- and inter-day assays, and the accuracy ranged from 89 to 108%. The extract recovery for the four triterpenoids and internal standard ranged from 95 to 67%, and the QC samples were found to be stable according to the results of the stability study. This is the first report on determination of the major triterpenoids in rat plasma after oral administration of G. lucidum extract and the results provided a firm basis for clarifying the pharmacological effect of G. lucidum and evaluating the clinical applications of this medicinal fungus.

Original languageEnglish (US)
Pages (from-to)389-396
Number of pages8
JournalBiomedical Chromatography
Volume21
Issue number4
DOIs
StatePublished - Apr 2007
Externally publishedYes

Fingerprint

Reishi
Pharmacokinetics
High performance liquid chromatography
Oral Administration
Rats
High Pressure Liquid Chromatography
Plasmas
Hydrochloric Acid
Methylene Chloride
Fungi
Calibration
Hydrocortisone
Assays
Flow rate
Pharmacology
Recovery
Wavelength

Keywords

  • Ganoderma lucidum
  • HPLC
  • Pharmacokinetics
  • Triterpenoids

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

Cite this

HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract. / Wang, Xiao-Ming; Liu, Rongxia; Sun, Jianghao; Guan, Shuhong; Yang, Min; Bi, Kaishun; Guo, Dean.

In: Biomedical Chromatography, Vol. 21, No. 4, 04.2007, p. 389-396.

Research output: Contribution to journalArticle

Wang, Xiao-Ming ; Liu, Rongxia ; Sun, Jianghao ; Guan, Shuhong ; Yang, Min ; Bi, Kaishun ; Guo, Dean. / HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract. In: Biomedical Chromatography. 2007 ; Vol. 21, No. 4. pp. 389-396.
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