Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin

Simona Corrao, Rita Anzalone, Melania Lo Iacono, Tiziana Gorgun, Antonino Di Stefano, Silvestro Ennio D'Anna, Bruno Balbi, Mauro Carone, Anna Sala, Davide Corona, Anna Maria Timperio, Lello Zolla, Felicia Farina, Everly Conway De Macario, Alberto J.L. Macario, Francesco Cappello, Giampiero La Rocca

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.

Original languageEnglish (US)
Article number140125
JournalOpen Biology
Volume4
Issue number10
DOIs
StatePublished - Oct 29 2014
Externally publishedYes

Fingerprint

Chaperonins
Smoke
Tobacco Products
Lung
Pulmonary diseases
Chronic Obstructive Pulmonary Disease
Mucous Membrane
Chaperonin 10
Mitochondria
DNA
Isoelectric Point
Fibroblasts
Organelles
Cytosol
Molecular Weight
Molecular weight
Cells
Cell Line
Neoplasms
Proteins

Keywords

  • Bronchial epithelial cells
  • COPD
  • Hsp10
  • Lung fibroblasts
  • Nuclear localization

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin. / Corrao, Simona; Anzalone, Rita; Iacono, Melania Lo; Gorgun, Tiziana; Stefano, Antonino Di; D'Anna, Silvestro Ennio; Balbi, Bruno; Carone, Mauro; Sala, Anna; Corona, Davide; Timperio, Anna Maria; Zolla, Lello; Farina, Felicia; De Macario, Everly Conway; Macario, Alberto J.L.; Cappello, Francesco; Rocca, Giampiero La.

In: Open Biology, Vol. 4, No. 10, 140125, 29.10.2014.

Research output: Contribution to journalArticle

Corrao, S, Anzalone, R, Iacono, ML, Gorgun, T, Stefano, AD, D'Anna, SE, Balbi, B, Carone, M, Sala, A, Corona, D, Timperio, AM, Zolla, L, Farina, F, De Macario, EC, Macario, AJL, Cappello, F & Rocca, GL 2014, 'Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin', Open Biology, vol. 4, no. 10, 140125. https://doi.org/10.1098/rsob.140125
Corrao, Simona ; Anzalone, Rita ; Iacono, Melania Lo ; Gorgun, Tiziana ; Stefano, Antonino Di ; D'Anna, Silvestro Ennio ; Balbi, Bruno ; Carone, Mauro ; Sala, Anna ; Corona, Davide ; Timperio, Anna Maria ; Zolla, Lello ; Farina, Felicia ; De Macario, Everly Conway ; Macario, Alberto J.L. ; Cappello, Francesco ; Rocca, Giampiero La. / Hsp10 nuclear localization and changes in lung cells response to cigarette smoke suggest novel roles for this chaperonin. In: Open Biology. 2014 ; Vol. 4, No. 10.
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abstract = "Heat-shock protein (Hsp)10 is the co-chaperone for Hsp60 inside mitochondria, but it also resides outside the organelle. Variations in its levels and intracellular distribution have been documented in pathological conditions, e.g. cancer and chronic obstructive pulmonary disease (COPD). Here, we show that Hsp10 in COPD undergoes changes at the molecular and subcellular levels in bronchial cells from human specimens and derived cell lines, intact or subjected to stress induced by cigarette smoke extract (CSE). Noteworthy findings are: (i) Hsp10 occurred in nuclei of epithelial and lamina propria cells of bronchial mucosa from non-smokers and smokers; (ii) human bronchial epithelial (16HBE) and lung fibroblast (HFL-1) cells, in vitro, showed Hsp10 in the nucleus, before and after CSE exposure; (iii) CSE stimulation did not increase the levels of Hsp10 but did elicit qualitative changes as indicated by molecular weight and isoelectric point shifts; and (iv) Hsp10 nuclear levels increased after CSE stimulation in HFL-1, indicating cytosol to nucleus migration, and although Hsp10 did not bind DNA, it bound a DNA-associated protein.",
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AU - Stefano, Antonino Di

AU - D'Anna, Silvestro Ennio

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AU - Sala, Anna

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AU - Zolla, Lello

AU - Farina, Felicia

AU - De Macario, Everly Conway

AU - Macario, Alberto J.L.

AU - Cappello, Francesco

AU - Rocca, Giampiero La

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