H2S, a Bacterial Defense Mechanism against the Host Immune Response

Tracy Toliver-Kinsky, Weihua Cui, Gabor Törö, Seung-Jin Lee, Konstantin Shatalin, Evgeny Nudler, Csaba Szabo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The biological mediator hydrogen sulfide (H2S) is produced by bacteria and has been shown to be cytoprotective against oxidative stress and to increase the sensitivity of various bacteria to a range of antibiotic drugs. Here we evaluated whether bacterial H2S provides resistance against the immune response, using two bacterial species that are common sources of nosocomial infections, Escherichia coli and Staphylococcus aureus Elevations in H2S levels increased the resistance of both species to immune-mediated killing. Clearances of infections with wild-type and genetically H2S-deficient E. coli and S. aureus were compared in vitro and in mouse models of abdominal sepsis and burn wound infection. Also, inhibitors of H2S-producing enzymes were used to assess bacterial killing by leukocytes. We found that inhibition of bacterial H2S production can increase the susceptibility of both bacterial species to rapid killing by immune cells and can improve bacterial clearance after severe burn, an injury that increases susceptibility to opportunistic infections. These findings support the role of H2S as a bacterial defense mechanism against the host response and implicate bacterial H2S inhibition as a potential therapeutic intervention in the prevention or treatment of infections.

Original languageEnglish (US)
JournalInfection and Immunity
Volume87
Issue number1
DOIs
StatePublished - Jan 1 2019

Fingerprint

Staphylococcus aureus
Escherichia coli
Bacteria
Hydrogen Sulfide
Opportunistic Infections
Wound Infection
Cross Infection
Infection
Sepsis
Oxidative Stress
Leukocytes
Anti-Bacterial Agents
Wounds and Injuries
Enzymes
Pharmaceutical Preparations
Therapeutics
In Vitro Techniques

Keywords

  • antibiotic resistance
  • burn
  • hydrogen sulfide
  • opportunistic infections

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

H2S, a Bacterial Defense Mechanism against the Host Immune Response. / Toliver-Kinsky, Tracy; Cui, Weihua; Törö, Gabor; Lee, Seung-Jin; Shatalin, Konstantin; Nudler, Evgeny; Szabo, Csaba.

In: Infection and Immunity, Vol. 87, No. 1, 01.01.2019.

Research output: Contribution to journalArticle

Toliver-Kinsky, Tracy ; Cui, Weihua ; Törö, Gabor ; Lee, Seung-Jin ; Shatalin, Konstantin ; Nudler, Evgeny ; Szabo, Csaba. / H2S, a Bacterial Defense Mechanism against the Host Immune Response. In: Infection and Immunity. 2019 ; Vol. 87, No. 1.
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