Abstract
Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 246-255 |
| Number of pages | 10 |
| Journal | Cell Metabolism |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 9 2016 |
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ASJC Scopus subject areas
- Physiology
- Molecular Biology
- Cell Biology
Cite this
Human and Mouse Brown Adipose Tissue Mitochondria Have Comparable UCP1 Function. / Porter, Craig; Herndon, David; Chondronikola, Maria; Chao, Tony; Annamalai, Palam; Bhattarai, Nisha; Saraf, Manish K.; Capek, Karel D.; Reidy, Paul T.; Daquinag, Alexes C.; Kolonin, Mikhail G.; Rasmussen, Blake; Borsheim, Elisabet; Toliver-Kinsky, Tracy; Sidossis, Labros S.
In: Cell Metabolism, Vol. 24, No. 2, 09.08.2016, p. 246-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human and Mouse Brown Adipose Tissue Mitochondria Have Comparable UCP1 Function
AU - Porter, Craig
AU - Herndon, David
AU - Chondronikola, Maria
AU - Chao, Tony
AU - Annamalai, Palam
AU - Bhattarai, Nisha
AU - Saraf, Manish K.
AU - Capek, Karel D.
AU - Reidy, Paul T.
AU - Daquinag, Alexes C.
AU - Kolonin, Mikhail G.
AU - Rasmussen, Blake
AU - Borsheim, Elisabet
AU - Toliver-Kinsky, Tracy
AU - Sidossis, Labros S.
PY - 2016/8/9
Y1 - 2016/8/9
N2 - Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.
AB - Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.
UR - http://www.scopus.com/inward/record.url?scp=84991080772&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84991080772&partnerID=8YFLogxK
U2 - 10.1016/j.cmet.2016.07.004
DO - 10.1016/j.cmet.2016.07.004
M3 - Article
C2 - 27508873
AN - SCOPUS:84991080772
VL - 24
SP - 246
EP - 255
JO - Cell Metabolism
JF - Cell Metabolism
SN - 1550-4131
IS - 2
ER -