Human carcinoid cell production of paracrine growth factors that can stimulate fibroblast and endothelial cell growth

R. Daniel Beauchamp, Courtney Townsend, Russette M. Lyons, Elizabeth A. Perkett, Courtney M. Townsend, Harold L. Moses

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

A serotonin-secreting human pancreatic carcinoid cell line (BON) is demonstrated to express transcripts for all three mammalian types of transforming growth factor β (TGFβ1, 2, and 3). Similarly, freshly excised carcinoid tumors from six patients were also found to express mRNA for all three of the type-β TGFs. Medium conditioned by BON cells had detectable TGFβ activity, although most of the activity was latent as determined by radioreceptor assay with and without prior acid treatment. However, nonactivated BON-conditioned medium stimulated DNA synthesis, soft agar growth, and an increase in TGFβ1 and fibronectin mRNA expression in AKR-2B fibroblasts. In addition, BON-conditioned medium had a potent endothelial cell growth-stimulatory activity. Since the TGFβs inhibit growth of endothelial cells, the presence of other growth factors was suspected. TGFα, c-sis, and basic fibroblast growth factor transcripts were also found to be expressed by the BON carcinoid cells. These data indicate that multiple peptide growth factors may have a paracrine role in the desmoplastic reaction accompanying carcinoid tumors.

Original languageEnglish (US)
Pages (from-to)5253-5260
Number of pages8
JournalCancer Research
Volume51
Issue number19
StatePublished - Oct 1 1991
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Beauchamp, R. D., Townsend, C., Lyons, R. M., Perkett, E. A., Townsend, C. M., & Moses, H. L. (1991). Human carcinoid cell production of paracrine growth factors that can stimulate fibroblast and endothelial cell growth. Cancer Research, 51(19), 5253-5260.