Human cardiotrophin-1: Protein and gene structure, biological and binding activities, and chromosomal localization

Diane Pennica, Todd A. Swanson, Kenneth J. Shaw, Wun Jing Kuang, Christa L. Gray, Barbara G. Beatty, William I. Wood

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Cardiotrophin-1 (CT-1) is a new member of the interleukin-6 cytokine family that was identified from a mouse embryoid body cDNA library by expression cloning. Mouse CT-1 induces features of hypertrophy in neonatal rat cardiac myocytes and binds to and activates the leukaemia inhibitory factor/gp130 receptor complex. In this work we report the isolation and characterization of cDNA and genomic clones encoding human CT-1. These clones encode a 201 amino acid protein that is 80% identical to the mouse protein. Human CT-1 produced by transfection of the cDNA clones into mammalian cells induces the hypertrophy of neonatal rat cardiac myocytes. Human and mouse CT-1 bind to the leukaemia inhibitory factor receptor on both human and mouse cell lines indicating a lack of species specificity. No binding to the human oncostatin M specific receptor was detected. A 1.7 kb CT-1 mRNA is expressed in adult human heart, skeletal muscle, ovary, colon, prostate and testis and in fetal kidney and lung. The coding region of CT-1 is contained on three exons and is located on human chromosome 16p11.1-16p11.2.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalCytokine
Volume8
Issue number3
DOIs
StatePublished - Mar 1996
Externally publishedYes

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Keywords

  • Cardiotrophin
  • cDNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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