Human CD8+ T cells clear Cryptosporidium parvum from infected intestinal epithelial cells

Birte Pantenburg, Alejandro Castellanos-Gonzalez, Sara M. Dann, Rhykka L. Connelly, Dorothy E. Lewis, Honorine D. Ward, A. Clinton White

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Intracellular protozoans of the genus Cryptosporidium are a major cause of diarrheal illness worldwide, especially in immunocompromised individuals. CD4+ T cells and interferon-gamma are key factors in the control of cryptosporidiosis in human and murine models. Previous studies led us to hypothesize that CD8+ T cells contribute to clearance of intestinal epithelial Cryptosporidium infection in humans. We report here that antigen expanded sensitized CD8+ T cells reduce the parasite load in infected intestinal epithelial cell cultures and lyse infected intestinal epithelial cells. These effects are most likely mediated by the release of cytotoxic granules. Elimination of parasites seems to require antigen presentation through both human leukocyte antigen (HLA)-A and HLA-B. These data suggest that cytotoxic CD8+ T cells play a role in clearing Cryptosporidium from the intestine, a previously unrecognized feature of the human immune response against this parasite.

Original languageEnglish (US)
Pages (from-to)600-607
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number4
DOIs
StatePublished - Apr 2010

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

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