Human endogenous retroviral K element encodes fusogenic activity in melanoma cells

Gengming Huang, Zhongwu Li, Xiaohua Wan, Yue Wang, Jianli Dong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Introduction and Hypothesis: Nuclear atypia with features of multi nuclei have been detected in human melanoma specimens. We found that the K type human endogenous retroviral element (HERV K) is expressed in such cells. Since cellular syncytia can form when cells are infected with retroviruses, we hypothesized that HERV K expressed in melanoma cells may contribute to the formation of multinuclear atypia cells in melanoma. Experiments and Results: We specifically inhibited HERV K expression using RNA interference (RNAi) and monoclonal antibodies and observed dramatic reduction of intercellular fusion of cultured melanoma cells. Importantly, we identified loss of heterozygosity (LOH)of D19S433 in a cell clone that survived and proliferated after cell fusion. Conclusion: Our results support the notion that proteins encoded by HERV K can mediate intercellular fusion of melanoma cells, which may generate multinuclear cells and drive the evolution of genetic changes that provide growth and survival advantages.

Original languageEnglish (US)
Article number109032
JournalJournal of Carcinogenesis
Volume12
DOIs
StatePublished - Jan 1 2013

Fingerprint

Endogenous Retroviruses
Melanoma
Cell Fusion
Molecular Evolution
Loss of Heterozygosity
Giant Cells
Retroviridae
RNA Interference
Cultured Cells
Clone Cells
Monoclonal Antibodies
Survival
Growth
Proteins

Keywords

  • Cell fusion
  • K-type human endogenous retroviral deoxyribonucleic acid
  • melanoma

ASJC Scopus subject areas

  • Cancer Research
  • Health, Toxicology and Mutagenesis
  • Oncology

Cite this

Human endogenous retroviral K element encodes fusogenic activity in melanoma cells. / Huang, Gengming; Li, Zhongwu; Wan, Xiaohua; Wang, Yue; Dong, Jianli.

In: Journal of Carcinogenesis, Vol. 12, 109032, 01.01.2013.

Research output: Contribution to journalArticle

Huang, Gengming ; Li, Zhongwu ; Wan, Xiaohua ; Wang, Yue ; Dong, Jianli. / Human endogenous retroviral K element encodes fusogenic activity in melanoma cells. In: Journal of Carcinogenesis. 2013 ; Vol. 12.
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