Human endothelial cell aminopeptidase P

H. Ju, N. D. Denslow, J. W. Ryan, Andreas Papapetropoulos, A. Antonov, R. Virmani, F. D. Kolodgie, R. G. Gerritv, J. D. Catravas

Research output: Contribution to journalArticle

Abstract

On sequencing peptides formed by LysC digests of guinea pig lung and kidney aminopeptidase P (AmP). we found peptide segments that are highly homologous with three of six conserved blocks characteristic of the protine peptidase family (PPF). It. as we postulate, AmP is a member of the PPF, the order of the conserved blocks (block A is most N-terminal, block F is most C-terminal) provides clues for the design of oligonucleotide primers for cloning a substantial portion of AmP cDNA. Thus, we prepared two forward and two reverse primers: N - representing a novel sequence believed to be closest to the cDNA 5 -end, C- and -C representing block C, and -EF representing parts of blocks E and F (which are virtually fused in AmP). We also used the 3 RACE kit APT primer. RT-PCR was performed using human aortic endothelial cell (HAEC) RNA. NK APT primers produced a 1,000 bp product; N-, -C yielded a 320 bp product; N-, -EF yielded a 630 bp product. We conclude that HAEC contain AmP mRNA. Judging from the MW of the AmP peptide, we have obtained the cDNA that encodes its C-terminal half plus an unlranslated peptide. The N-, -EF segment contains about 200 amino acid residues; the N-, -C segment contains about 100 residues; F is followed by about 100 residues, some probably removed in translation.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Endothelial cells
Endothelial Cells
Peptides
Complementary DNA
Peptide Hydrolases
DNA Primers
Cloning
X-Pro aminopeptidase
Organism Cloning
Guinea Pigs
RNA
Kidney
Amino Acids
Polymerase Chain Reaction
Lung
Messenger RNA

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Ju, H., Denslow, N. D., Ryan, J. W., Papapetropoulos, A., Antonov, A., Virmani, R., ... Catravas, J. D. (1996). Human endothelial cell aminopeptidase P. FASEB Journal, 10(3).

Human endothelial cell aminopeptidase P. / Ju, H.; Denslow, N. D.; Ryan, J. W.; Papapetropoulos, Andreas; Antonov, A.; Virmani, R.; Kolodgie, F. D.; Gerritv, R. G.; Catravas, J. D.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Ju, H, Denslow, ND, Ryan, JW, Papapetropoulos, A, Antonov, A, Virmani, R, Kolodgie, FD, Gerritv, RG & Catravas, JD 1996, 'Human endothelial cell aminopeptidase P', FASEB Journal, vol. 10, no. 3.
Ju H, Denslow ND, Ryan JW, Papapetropoulos A, Antonov A, Virmani R et al. Human endothelial cell aminopeptidase P. FASEB Journal. 1996 Dec 1;10(3).
Ju, H. ; Denslow, N. D. ; Ryan, J. W. ; Papapetropoulos, Andreas ; Antonov, A. ; Virmani, R. ; Kolodgie, F. D. ; Gerritv, R. G. ; Catravas, J. D. / Human endothelial cell aminopeptidase P. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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