Human endothelial cell culture plaques induced by Rickettsia rickettsii

David Walker, W. T. Firth, C. J S Edgell

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Primary cultures of human umbilical vein endothelial cells were inoculated with plaque-purified Rickettsia rickettsii. After adsorption of rickettsiae, monolayers were overlaid with medium containing 0.5% agarose. Small plaques appeared on day 4 postinoculation, and distinct 1- to 2-mm plaques were observed on day 5. Plaquing efficiency was less than that of primary chicken embryo cells in the same medium. Human endothelial cell monolayers were susceptible to infection by R. ricketsii and underwent necrosis as demonstrated by supravital staining. The topographic association of endothelial cell necrosis and rickettsial infection in the plaque model confirmed the direct cytopathic effect of R. rickettsii on human endothelium. Uninfected cells appeared normal by supravital staining and transmission electron microscopy. This model offers the possibility of investigating rickettsial pathogenesis and mechanisms of enhanced severity of Rocky Mountain spotted fever in specific genetically determined conditions.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalInfection and Immunity
Volume37
Issue number1
StatePublished - 1982
Externally publishedYes

Fingerprint

Rickettsia rickettsii
Necrosis
Endothelial Cells
Cell Culture Techniques
Rocky Mountain Spotted Fever
Staining and Labeling
Rickettsia
Human Umbilical Vein Endothelial Cells
Infection
Transmission Electron Microscopy
Sepharose
Adsorption
Endothelium
Chickens
Embryonic Structures

ASJC Scopus subject areas

  • Immunology

Cite this

Human endothelial cell culture plaques induced by Rickettsia rickettsii. / Walker, David; Firth, W. T.; Edgell, C. J S.

In: Infection and Immunity, Vol. 37, No. 1, 1982, p. 301-306.

Research output: Contribution to journalArticle

Walker, David ; Firth, W. T. ; Edgell, C. J S. / Human endothelial cell culture plaques induced by Rickettsia rickettsii. In: Infection and Immunity. 1982 ; Vol. 37, No. 1. pp. 301-306.
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