Human febrile illness caused by encephalomyocarditis virus infection, peru

M. Steven Oberste, Eduardo Gotuzzo, Patrick Blair, W. Allan Nix, Thomas Ksiazek, James A. Comer, Pierre Rollin, Cynthia S. Goldsmith, James Olson, Tadeusz J. Kochel

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients' clinical signs and symptoms were recorded, and acute and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription-PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness.

Original languageEnglish (US)
Pages (from-to)640-646
Number of pages7
JournalEmerging Infectious Diseases
Volume15
Issue number4
DOIs
StatePublished - Apr 2009
Externally publishedYes

Fingerprint

Encephalomyocarditis virus
Peru
Virus Diseases
Fever
Cardiovirus
Viruses
Serum
Molecular Diagnostic Techniques
Vero Cells
Antibodies
South America
Acute Disease
Dyspnea
Nausea
Reverse Transcription
Signs and Symptoms
Immunoglobulin M
Headache
Electron Microscopy
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

Cite this

Oberste, M. S., Gotuzzo, E., Blair, P., Nix, W. A., Ksiazek, T., Comer, J. A., ... Kochel, T. J. (2009). Human febrile illness caused by encephalomyocarditis virus infection, peru. Emerging Infectious Diseases, 15(4), 640-646. https://doi.org/10.3201/eid1504.081428

Human febrile illness caused by encephalomyocarditis virus infection, peru. / Oberste, M. Steven; Gotuzzo, Eduardo; Blair, Patrick; Nix, W. Allan; Ksiazek, Thomas; Comer, James A.; Rollin, Pierre; Goldsmith, Cynthia S.; Olson, James; Kochel, Tadeusz J.

In: Emerging Infectious Diseases, Vol. 15, No. 4, 04.2009, p. 640-646.

Research output: Contribution to journalArticle

Oberste, MS, Gotuzzo, E, Blair, P, Nix, WA, Ksiazek, T, Comer, JA, Rollin, P, Goldsmith, CS, Olson, J & Kochel, TJ 2009, 'Human febrile illness caused by encephalomyocarditis virus infection, peru', Emerging Infectious Diseases, vol. 15, no. 4, pp. 640-646. https://doi.org/10.3201/eid1504.081428
Oberste, M. Steven ; Gotuzzo, Eduardo ; Blair, Patrick ; Nix, W. Allan ; Ksiazek, Thomas ; Comer, James A. ; Rollin, Pierre ; Goldsmith, Cynthia S. ; Olson, James ; Kochel, Tadeusz J. / Human febrile illness caused by encephalomyocarditis virus infection, peru. In: Emerging Infectious Diseases. 2009 ; Vol. 15, No. 4. pp. 640-646.
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