Human hepatitis δ virus RNA subfragments contain an autocleavage activity

H. N. Wu, Y. J. Lin, F. P. Lin, S. Makino, M. F. Chang, M. M.C. Lai

Research output: Contribution to journalArticle

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Abstract

Hepatitis δ virus (HDV) contains a single-stranded circular RNA genome of 1.7 kilobases. In this report we demonstrate that subfragments of HDV RNA can undergo autocatalytic cleavage. This cleavage requires at least 500 μM of Mg2+ or Ca2+, is not affected by varying the pH from 5.0 to 9.1, and occurs with RNA fragments as small as 133 nucleotides. The larger RNA fragments containing additional HDV sequences have a lower efficiency of cleavage. Deletion analysis at both ends of RNA subfragments suggested that the catalytic ability of HDV RNA resides in a stretch of not more than 117 nucleotides around the cleavage site. The cleavage occurs at the phosphodiester bond between nucleotides 688 and 689 on the HDV genomic map, generating a 5' fragment with a terminal uridyl 2',3'-cyclic monophosphate residue and a 3' fragment with a guanosyl residue with a 5'-hydroxyl group. The smallest autocleaving RNA does not contain the 'hammerhead' sequence required for the autocleavage of other known self-cleaving RNA. The cleavage of HDV RNA occurs at a much faster rate, even at a very low Mg2+ concentration, than that of other 'ribozymes'. Thus, HDV RNA represents a distinct class of ribozyme.

Original languageEnglish (US)
Pages (from-to)1831-1835
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number6
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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