Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability

Britney Johnson, Laura A. VanBlargan, Wei Xu, James P. White, Chao Shan, Pei-Yong Shi, Rong Zhang, Jagat Adhikari, Michael L. Gross, Daisy W. Leung, Michael S. Diamond, Gaya K. Amarasinghe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Although interferon-induced proteins with tetratricopeptide repeats (IFIT proteins) inhibit infection of many viruses by recognizing their RNA, the regulatory mechanisms involved remain unclear. Here we report a crystal structure of cap 0 (m7GpppN) RNA bound to human IFIT1 in complex with the C-terminal domain of human IFIT3. Structural, biochemical, and genetic studies suggest that IFIT3 binding to IFIT1 has dual regulatory functions: (1) extending the half-life of IFIT1 and thereby increasing its steady-state amounts in cells; and (2) allosterically regulating the IFIT1 RNA-binding channel, thereby enhancing the specificity of recognition for cap 0 but not cap 1 (m7GpppNm) or 5′-ppp RNA. Mouse Ifit3 lacks this key C-terminal domain and does not bind mouse Ifit1. The IFIT3 interaction with IFIT1 is important for restricting infection of viruses lacking 2′-O methylation in their RNA cap structures. Our experiments establish differences in the regulation of IFIT1 orthologs and define targets for modulation of human IFIT protein activity. Prior studies have suggested that human IFIT1, unlike its mouse ortholog, might not recognize viral RNA molecules lacking 2′-O methylation on their cap structures. Johnson et al. report a crystal structure between cap 0 (m7GpppN) RNA bound to human IFIT1 in complex with the C-terminal domain (CTD) of human IFIT3. The CTD of IFIT3 bound to IFIT1 and allosterically regulated the IFIT1 RNA-binding channel and promoted selective recognition of cap 0 RNA. Functional studies demonstrated that IFIT3 interaction with IFIT1 was important for stabilizing IFIT1 expression and was required for restricting infection of viruses lacking 2′-O methylation in their RNA cap structures

Original languageEnglish (US)
Pages (from-to)487-499.e5
JournalImmunity
Volume48
Issue number3
DOIs
StatePublished - Mar 20 2018

Fingerprint

RNA-Binding Proteins
Protein Stability
RNA Caps
RNA
Virus Diseases
Methylation
Proteins
Viral RNA
Interferons
Half-Life
Molecular Biology

Keywords

  • antiviral
  • crystallography
  • flavivirus
  • host defense
  • innate immunity
  • interferon stimulated genes
  • pathogen associated molecular patterns
  • RNA-binding protein
  • self/non-self RNA recognition
  • X-ray

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Johnson, B., VanBlargan, L. A., Xu, W., White, J. P., Shan, C., Shi, P-Y., ... Amarasinghe, G. K. (2018). Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. Immunity, 48(3), 487-499.e5. https://doi.org/10.1016/j.immuni.2018.01.014

Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. / Johnson, Britney; VanBlargan, Laura A.; Xu, Wei; White, James P.; Shan, Chao; Shi, Pei-Yong; Zhang, Rong; Adhikari, Jagat; Gross, Michael L.; Leung, Daisy W.; Diamond, Michael S.; Amarasinghe, Gaya K.

In: Immunity, Vol. 48, No. 3, 20.03.2018, p. 487-499.e5.

Research output: Contribution to journalArticle

Johnson, B, VanBlargan, LA, Xu, W, White, JP, Shan, C, Shi, P-Y, Zhang, R, Adhikari, J, Gross, ML, Leung, DW, Diamond, MS & Amarasinghe, GK 2018, 'Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability', Immunity, vol. 48, no. 3, pp. 487-499.e5. https://doi.org/10.1016/j.immuni.2018.01.014
Johnson B, VanBlargan LA, Xu W, White JP, Shan C, Shi P-Y et al. Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. Immunity. 2018 Mar 20;48(3):487-499.e5. https://doi.org/10.1016/j.immuni.2018.01.014
Johnson, Britney ; VanBlargan, Laura A. ; Xu, Wei ; White, James P. ; Shan, Chao ; Shi, Pei-Yong ; Zhang, Rong ; Adhikari, Jagat ; Gross, Michael L. ; Leung, Daisy W. ; Diamond, Michael S. ; Amarasinghe, Gaya K. / Human IFIT3 Modulates IFIT1 RNA Binding Specificity and Protein Stability. In: Immunity. 2018 ; Vol. 48, No. 3. pp. 487-499.e5.
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