Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells

Mark Endsley, Leo M. Njongmeta, Elisabeth Shell, Matthew W. Ryan, Alexander J. Indrikovs, Seckin Ulualp, Randall M. Goldblum, Waithaka Mwangi, D. Mark Estes

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Over the last several years, there has been a great deal of progress in characterizing the role of dendritic cells (DCs) in the activation and modulation of B cells. DC-secreted chemokines can induce B cell trafficking to the lymph nodes. DC-produced survival factors such as B cell-activating factor of the TNF family and a proliferation-inducing ligand have been shown to be essential for B cell maturation, but have also been implicated in class-switch recombination and B cell lymphoma survival. Recently added to this list of DC-derived factors effecting B cells is IgA-inducing protein (IGIP). In this study, we characterize production of IGIP by human DCs, and examine its capacity to induce IgA class switching and differentiation of naive B cells in vitro. Monocyte-derived DCs were cultured in vitro with TLR agonists (TLR3, 4, 5, and 9) and other factors, including CD40 ligand, GM-CSF, and IL-4 as well as the neuropeptide vasoactive intestinal peptide. Under in vitro stimulation with vasoactive intestinal peptide and CD40L, IGIP mRNA expression could be up-regulated as much as 35-fold above nonstimulated samples within 12-48 h. Naive B cells cultured with exogenous recombinant human IGIP produced IgA in greater quantities than nonstimulated controls. Finally, we demonstrate that IGIP stimulation drives the production of μ-α switch circles from IgM+IgD+ naive human B cells, indicating its role as an IgA switch factor.

Original languageEnglish (US)
Pages (from-to)1854-1859
Number of pages6
JournalJournal of Immunology
Volume182
Issue number4
DOIs
StatePublished - Feb 15 2009

Fingerprint

Immunoglobulin D
Dendritic Cells
Immunoglobulin A
B-Lymphocytes
CD40 Ligand
Vasoactive Intestinal Peptide
Cell Survival
B-Cell Activating Factor
Immunoglobulin Class Switching
Proteins
B-Cell Lymphoma
Granulocyte-Macrophage Colony-Stimulating Factor
Human IgA-inducing protein
Neuropeptides
Chemokines
Interleukin-4
Genetic Recombination
Immunoglobulin M
Monocytes
Lymph Nodes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Endsley, M., Njongmeta, L. M., Shell, E., Ryan, M. W., Indrikovs, A. J., Ulualp, S., ... Estes, D. M. (2009). Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells. Journal of Immunology, 182(4), 1854-1859. https://doi.org/10.4049/jimmunol.0801973

Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells. / Endsley, Mark; Njongmeta, Leo M.; Shell, Elisabeth; Ryan, Matthew W.; Indrikovs, Alexander J.; Ulualp, Seckin; Goldblum, Randall M.; Mwangi, Waithaka; Estes, D. Mark.

In: Journal of Immunology, Vol. 182, No. 4, 15.02.2009, p. 1854-1859.

Research output: Contribution to journalArticle

Endsley, M, Njongmeta, LM, Shell, E, Ryan, MW, Indrikovs, AJ, Ulualp, S, Goldblum, RM, Mwangi, W & Estes, DM 2009, 'Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells', Journal of Immunology, vol. 182, no. 4, pp. 1854-1859. https://doi.org/10.4049/jimmunol.0801973
Endsley, Mark ; Njongmeta, Leo M. ; Shell, Elisabeth ; Ryan, Matthew W. ; Indrikovs, Alexander J. ; Ulualp, Seckin ; Goldblum, Randall M. ; Mwangi, Waithaka ; Estes, D. Mark. / Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells. In: Journal of Immunology. 2009 ; Vol. 182, No. 4. pp. 1854-1859.
@article{5b6c4460007d4c3599e945c2d802ebf4,
title = "Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells",
abstract = "Over the last several years, there has been a great deal of progress in characterizing the role of dendritic cells (DCs) in the activation and modulation of B cells. DC-secreted chemokines can induce B cell trafficking to the lymph nodes. DC-produced survival factors such as B cell-activating factor of the TNF family and a proliferation-inducing ligand have been shown to be essential for B cell maturation, but have also been implicated in class-switch recombination and B cell lymphoma survival. Recently added to this list of DC-derived factors effecting B cells is IgA-inducing protein (IGIP). In this study, we characterize production of IGIP by human DCs, and examine its capacity to induce IgA class switching and differentiation of naive B cells in vitro. Monocyte-derived DCs were cultured in vitro with TLR agonists (TLR3, 4, 5, and 9) and other factors, including CD40 ligand, GM-CSF, and IL-4 as well as the neuropeptide vasoactive intestinal peptide. Under in vitro stimulation with vasoactive intestinal peptide and CD40L, IGIP mRNA expression could be up-regulated as much as 35-fold above nonstimulated samples within 12-48 h. Naive B cells cultured with exogenous recombinant human IGIP produced IgA in greater quantities than nonstimulated controls. Finally, we demonstrate that IGIP stimulation drives the production of μ-α switch circles from IgM+IgD+ naive human B cells, indicating its role as an IgA switch factor.",
author = "Mark Endsley and Njongmeta, {Leo M.} and Elisabeth Shell and Ryan, {Matthew W.} and Indrikovs, {Alexander J.} and Seckin Ulualp and Goldblum, {Randall M.} and Waithaka Mwangi and Estes, {D. Mark}",
year = "2009",
month = "2",
day = "15",
doi = "10.4049/jimmunol.0801973",
language = "English (US)",
volume = "182",
pages = "1854--1859",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Human IgA-inducing protein from dendritic cells induces IgA production by naive IgD+ B cells

AU - Endsley, Mark

AU - Njongmeta, Leo M.

AU - Shell, Elisabeth

AU - Ryan, Matthew W.

AU - Indrikovs, Alexander J.

AU - Ulualp, Seckin

AU - Goldblum, Randall M.

AU - Mwangi, Waithaka

AU - Estes, D. Mark

PY - 2009/2/15

Y1 - 2009/2/15

N2 - Over the last several years, there has been a great deal of progress in characterizing the role of dendritic cells (DCs) in the activation and modulation of B cells. DC-secreted chemokines can induce B cell trafficking to the lymph nodes. DC-produced survival factors such as B cell-activating factor of the TNF family and a proliferation-inducing ligand have been shown to be essential for B cell maturation, but have also been implicated in class-switch recombination and B cell lymphoma survival. Recently added to this list of DC-derived factors effecting B cells is IgA-inducing protein (IGIP). In this study, we characterize production of IGIP by human DCs, and examine its capacity to induce IgA class switching and differentiation of naive B cells in vitro. Monocyte-derived DCs were cultured in vitro with TLR agonists (TLR3, 4, 5, and 9) and other factors, including CD40 ligand, GM-CSF, and IL-4 as well as the neuropeptide vasoactive intestinal peptide. Under in vitro stimulation with vasoactive intestinal peptide and CD40L, IGIP mRNA expression could be up-regulated as much as 35-fold above nonstimulated samples within 12-48 h. Naive B cells cultured with exogenous recombinant human IGIP produced IgA in greater quantities than nonstimulated controls. Finally, we demonstrate that IGIP stimulation drives the production of μ-α switch circles from IgM+IgD+ naive human B cells, indicating its role as an IgA switch factor.

AB - Over the last several years, there has been a great deal of progress in characterizing the role of dendritic cells (DCs) in the activation and modulation of B cells. DC-secreted chemokines can induce B cell trafficking to the lymph nodes. DC-produced survival factors such as B cell-activating factor of the TNF family and a proliferation-inducing ligand have been shown to be essential for B cell maturation, but have also been implicated in class-switch recombination and B cell lymphoma survival. Recently added to this list of DC-derived factors effecting B cells is IgA-inducing protein (IGIP). In this study, we characterize production of IGIP by human DCs, and examine its capacity to induce IgA class switching and differentiation of naive B cells in vitro. Monocyte-derived DCs were cultured in vitro with TLR agonists (TLR3, 4, 5, and 9) and other factors, including CD40 ligand, GM-CSF, and IL-4 as well as the neuropeptide vasoactive intestinal peptide. Under in vitro stimulation with vasoactive intestinal peptide and CD40L, IGIP mRNA expression could be up-regulated as much as 35-fold above nonstimulated samples within 12-48 h. Naive B cells cultured with exogenous recombinant human IGIP produced IgA in greater quantities than nonstimulated controls. Finally, we demonstrate that IGIP stimulation drives the production of μ-α switch circles from IgM+IgD+ naive human B cells, indicating its role as an IgA switch factor.

UR - http://www.scopus.com/inward/record.url?scp=61449089447&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61449089447&partnerID=8YFLogxK

U2 - 10.4049/jimmunol.0801973

DO - 10.4049/jimmunol.0801973

M3 - Article

VL - 182

SP - 1854

EP - 1859

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -