Human immunodeficiency virus infection induces lymphoid fibrosis in the BM-liver-thymus-spleen humanized mouse model

Jasmine Samal, Samantha Kelly, Ali Na-Shatal, Abdallah Elhakiem, Antu Das, Ming Ding, Anwesha Sanyal, Phalguni Gupta, Kevin Melody, Brad Roland, Watfa Ahmed, Aala Zakir, Moses Bility

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A major pathogenic feature associated with HIV infection is lymphoid fibrosis, which persists during antiretroviral therapy (ART). Lymphoid tissues play critical roles in the generation of antigen-specific immune response, and fibrosis disrupts the stromal network of lymphoid tissues, resulting in impaired immune cell trafficking and function, as well as immunodeficiency. Developing an animal model for investigating the impact of HIV infection-induced lymphoid tissue fibrosis on immunodeficiency and immune cell impairment is critical for therapeutics development and clinical translation. Said model will enable in vivo mechanistic studies, thus complementing the well-established surrogate model of SIV infection-induced lymphoid tissue fibrosis in macaques. We developed a potentially novel human immune system-humanized mouse model by coengrafting autologous fetal thymus, spleen, and liver organoids under the kidney capsule, along with i.v. injection of autologous fetal liver-derived hematopoietic stem cells, thus termed the BM-liver-thymus-spleen (BLTS) humanized mouse model. BLTS humanized mouse model supports development of human immune cells and human lymphoid organoids (human thymus and spleen organoids). HIV infection in BLTS humanized mice results in progressive fibrosis in human lymphoid tissues, which was associated with immunodeficiency in the lymphoid tissues, and lymphoid tissue fibrosis persists during ART, thus recapitulating clinical outcomes.

Original languageEnglish (US)
JournalJCI insight
Volume3
Issue number18
DOIs
StatePublished - Sep 20 2018
Externally publishedYes

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Lymphoid Tissue
Virus Diseases
Thymus Gland
Fibrosis
Spleen
HIV
Organoids
Liver
HIV Infections
Histocompatibility Antigens Class II
Macaca
Human Development
Hematopoietic Stem Cells
Capsules
Immune System
Therapeutics
Animal Models
Lymphocytes
Kidney
Injections

Keywords

  • AIDS/HIV
  • Fibrosis
  • Mouse models
  • Organogenesis

Cite this

Human immunodeficiency virus infection induces lymphoid fibrosis in the BM-liver-thymus-spleen humanized mouse model. / Samal, Jasmine; Kelly, Samantha; Na-Shatal, Ali; Elhakiem, Abdallah; Das, Antu; Ding, Ming; Sanyal, Anwesha; Gupta, Phalguni; Melody, Kevin; Roland, Brad; Ahmed, Watfa; Zakir, Aala; Bility, Moses.

In: JCI insight, Vol. 3, No. 18, 20.09.2018.

Research output: Contribution to journalArticle

Samal, J, Kelly, S, Na-Shatal, A, Elhakiem, A, Das, A, Ding, M, Sanyal, A, Gupta, P, Melody, K, Roland, B, Ahmed, W, Zakir, A & Bility, M 2018, 'Human immunodeficiency virus infection induces lymphoid fibrosis in the BM-liver-thymus-spleen humanized mouse model', JCI insight, vol. 3, no. 18. https://doi.org/10.1172/jci.insight.120430
Samal, Jasmine ; Kelly, Samantha ; Na-Shatal, Ali ; Elhakiem, Abdallah ; Das, Antu ; Ding, Ming ; Sanyal, Anwesha ; Gupta, Phalguni ; Melody, Kevin ; Roland, Brad ; Ahmed, Watfa ; Zakir, Aala ; Bility, Moses. / Human immunodeficiency virus infection induces lymphoid fibrosis in the BM-liver-thymus-spleen humanized mouse model. In: JCI insight. 2018 ; Vol. 3, No. 18.
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