Human immunodeficiency virus type 1 Vpu modifies viral cytopathic effect through augmented virus release

Yasumasa Iwatani, Seong K. Song, Liqiang Wang, Jose Planas, Hiroyuki Sakai, Akinori Ishimoto, Miles W. Cloyd

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We characterized two human immunodeficiency virus type 1 (HIV-1) strains, HIV(MCK) and HIV213, which have different cytopathic effects in infected cells. HIV213 was highly cytopathic, whereas HIV(MCK) was not. Biological analyses of chimeric viruses from the cloned infectious DNAs of HIV(MCK) and HIV213 showed that the Vpu region was responsible for the differing cytopathicity of these viruses. Although HIV(MCK) expressed Vpu protein, HIV213 did not because of a mutation at the start codon for Vpu. The amounts of envelope glycoprotein and virus particles associated with the cell surface were significantly increased on cells infected with Vpu-deficient viruses compared with Vpu-positive viruses. These data suggest that the highly cytopathic effects of HIV213 (Vpu-deficient) are due to an accumulation of envelope glycoprotein at the infected-cell surface, which would be caused by the retention of progeny virions in the absence of Vpu-facilitated virion release.

Original languageEnglish (US)
Pages (from-to)841-846
Number of pages6
JournalJournal of General Virology
Volume78
Issue number4
DOIs
StatePublished - Apr 1997

ASJC Scopus subject areas

  • Virology

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    Iwatani, Y., Song, S. K., Wang, L., Planas, J., Sakai, H., Ishimoto, A., & Cloyd, M. W. (1997). Human immunodeficiency virus type 1 Vpu modifies viral cytopathic effect through augmented virus release. Journal of General Virology, 78(4), 841-846. https://doi.org/10.1099/0022-1317-78-4-841