Human lymphocyte production of immunoreactive thyrotropin

E. M. Smith, M. Phan, T. E. Kruger, D. H. Coppenhaver, J. E. Blalock

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Interferon-α inducers were previously shown to cause human lymphocyte production of a corticotropin (ACTH)-like peptide. Thyrotropin (TSH) was not produced under these conditions. In contrast, this report shows that a T-cell mitogen (staphylococcal enterotoxin A) which does not induce the ACTH-like peptide, caused human lymphocytes production of an immunoreactive (ir) TSH. Lymphocyte synthesis of the ir TSH was first detectable at 24 hr, peaked at 48 hr, and thereafter declined. NaDodSO4/polyacrylamide gel electrophoresis of intrinsically radiolabeled lymphocyte-derived ir TSH showed radiolabeled peaks at 80, 50, and 26 kilodaltons. These peaks presumably correspond to trimeric, dimeric, and monomeric TSH-like proteins, respectively. Acid treatment and reduction caused the ir TSH to migrate as a 14-kilodalton peak with a 12-kilodalton shoulder in a gel filtration column run in 6 M guanidine·HCl. Thus, the ir TSH seemed to be composed of subunits with molecular masses corresponding to those of the β and α chains of human TSH, respectively. The ir TSH appeared to be glycoprotein because it bound to a concanavalin A affinity column. Taken together these data suggest that in addition to ACTH, human lymphocytes can also produce a TSH-like substance.

Original languageEnglish
Pages (from-to)6010-6013
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number19 I
StatePublished - 1983

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Thyrotropin
Adrenocorticotropic Hormone
Lymphocytes
Interferon Inducers
Peptides
Concanavalin A
Mitogens
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Glycoproteins
T-Lymphocytes
Acids
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Smith, E. M., Phan, M., Kruger, T. E., Coppenhaver, D. H., & Blalock, J. E. (1983). Human lymphocyte production of immunoreactive thyrotropin. Proceedings of the National Academy of Sciences of the United States of America, 80(19 I), 6010-6013.

Human lymphocyte production of immunoreactive thyrotropin. / Smith, E. M.; Phan, M.; Kruger, T. E.; Coppenhaver, D. H.; Blalock, J. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, No. 19 I, 1983, p. 6010-6013.

Research output: Contribution to journalArticle

Smith, EM, Phan, M, Kruger, TE, Coppenhaver, DH & Blalock, JE 1983, 'Human lymphocyte production of immunoreactive thyrotropin', Proceedings of the National Academy of Sciences of the United States of America, vol. 80, no. 19 I, pp. 6010-6013.
Smith, E. M. ; Phan, M. ; Kruger, T. E. ; Coppenhaver, D. H. ; Blalock, J. E. / Human lymphocyte production of immunoreactive thyrotropin. In: Proceedings of the National Academy of Sciences of the United States of America. 1983 ; Vol. 80, No. 19 I. pp. 6010-6013.
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